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Human GRK2 / ADRBK1 Protein (His & GST Tag)

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Human ADRBK1 Protein Product Information
Synonym:ADRBK1, BARK1, BETA-ARK1, FLJ16718, GRK2
Protein Construction:A DNA sequence encoding the human ADRBK1 (NP_001610.2) (Met 1-Leu 689) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Expressed Host:Baculovirus-Insect Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human ADRBK1 Protein QC Testing
Purity:> 90 % as determined by SDS-PAGE
Bio-Activity:No Kinase Activity
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant human ADRBK1/GST chimera consists of 926 amino acids and has a calculated molecular mass of 107 kDa. It migrates as an approximately 110 kDa band in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile 50mM Tris, 500mM NaCl, 0.5mM GSH, pH 8.0
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human ADRBK1 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human ADRBK1 Protein SDS-PAGE
Human GRK2 / ADRBK1 Protein (His & GST Tag) SDS-PAGE
Human GRK2 / ADRBK1 Protein (His & GST Tag) SDS-PAGE
Other ADRBK1 Recombinant Protein Products
GRK2 / ADRBK1 Background

G-protein coupled receptor kinase 2 (GRK2), also referred as Adrenergic, beta, receptor kinase 1 (ADRBK1), is a ubiquitous member of the G protein-coupled receptor kinase (GRK) family that appears to play a central, integrative role in signal transduction cascades. GRK2 can phosphorylate a growing number of non-GPCR substrates and associate with a variety of proteins related to signal transduction, thus suggesting that this kinase could also have diverse 'effector' functions. GRK2 has been reported to interact with a variety of signal transduction proteins related to cell migration such as MEK, Akt, PI3Kgamma or GIT. Interestingly, the levels of expression and activity of this kinase are altered in a number of inflammatory disorders (as rheumatoid arthritis or multiple sclerosis), thus suggesting that GRK2 may play an important role in the onset or development of these pathologies. The important physiological function of GRK2 as a modulator of the efficacy of GPCR signal transduction systems is exemplified by its relevance in cardiovascular physiopathology as well as by its emerging role in the regulation of chemokine receptors. Besides its canonical role in the modulation of the signalling mediated by many G protein-coupled receptors (GPCR), this protein can display a very complex network of functional interactions with a variety of signal transduction partners, in a stimulus, cell type, or context-specific way.

Human GRK2 / ADRBK1 References
  • Penela P, et al. (2010) The complex G protein-coupled receptor kinase 2 (GRK2) interactome unveils new physiopathological targets. Br J Pharmacol. 160(4): 821-32.
  • Penela P, et al. (2008) G protein-coupled receptor kinase 2 (GRK2) in migration and inflammation. Arch Physiol Biochem. 114(3): 195-200.
  • Aragay AM, et al. (1998) protein-coupled receptor kinase 2 (GRK2): mechanisms of regulation and physiological functions. FEBS Lett. 430(1-2): 37-40.
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    Catalog: 11755-H20B-20
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    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.