|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human OTUB1 ORF mammalian expression plasmid, C-GFPSpark tag||HG16169-ACG|
|Human OTUB1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG16169-ACR|
|Human OTUB1 ORF mammalian expression plasmid, N-GFPSpark tag||HG16169-ANG|
|Human OTUB1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG16169-ANR|
|Human OTUB1 ORF mammalian expression plasmid, C-Flag tag||HG16169-CF|
|Human OTUB1 ORF mammalian expression plasmid, C-His tag||HG16169-CH|
|Human OTUB1 ORF mammalian expression plasmid, C-Myc tag||HG16169-CM|
|Human OTUB1 ORF mammalian expression plasmid, C-HA tag||HG16169-CY|
|Human OTUB1 Gene cDNA clone plasmid||HG16169-G|
|Human OTUB1 ORF mammalian expression plasmid, N-Flag tag||HG16169-NF|
|Human OTUB1 ORF mammalian expression plasmid, N-His tag||HG16169-NH|
|Human OTUB1 ORF mammalian expression plasmid, N-Myc tag||HG16169-NM|
|Human OTUB1 ORF mammalian expression plasmid, N-HA tag||HG16169-NY|
|Human OTUB1 Gene cDNA clone plasmid||HG16169-U|
|Human OTUB1 natural ORF mammalian expression plasmid||HG16169-UT|
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Ubiquitin thioesterase OTUB1, also known as Deubiquitinating enzyme OTUB1, OTU domain-containing ubiquitin aldehyde-binding protein 1, Otubain-1, Ubiquitin-specific-processing protease OTUB1, OTUB1 and OTB1, is a cytoplasm protein which belongs to the peptidase C65 family. OTUB1 is a hydrolase that can remove conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. OTUB1 is a regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. OTUB1 acts via its interaction with RNF128 / GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 of OTUB1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 of OTUB1 stabilizes RNF128 and promotes anergy. OTUB1 regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). OTUB1 mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. OTUB1 is also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin.