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VNN2 / Vanin-2 Antibody, Mouse MAb

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Human VNN2 Antibody Product Information
Immunogen:Recombinant Human VNN2 protein (Catalog#11728-H08H)
Clone ID:3F8F8H6
Ig Type:Mouse IgG1
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human VNN2 / Vanin-2 (rh VNN2 / Vanin-2; Catalog#11728-H08H; NP_004656.2; Met 1-Ser 492). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human VNN2 Antibody Usage Guide
Specificity:Human VNN2 / Vanin-2
No cross-reactivity in ELISA with
Human cell lysate (293 cell line)
Application:ELISA, FCM

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human VNN2. The detection limit for Human VNN2 is approximately 0.078 ng/well.

FCM: 0.5-2 μg/Test

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human VNN2 Antibody FC Application Image
[Click to enlarge image]
Flow cytometric analysis of Human VNN2 expression on human whole blood monocytes (left panel) and granulocytes (right panel). Cells were stained with purified anti-Human VNN2, then a FITC-conjugated second step antibody. The histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes and granulocytes.
VNN2 / Vanin-2 Antibody, Mouse MAb, Flow cytometric analysis
Other VNN2 Antibody Products
VNN2/Vanin-2 Background

Vascular non-inflammatory molecule 2, also known as glycosyl-phosphatidyl inositol-anchored protein GPI-80, Vanin-2, Protein FOAP-4 and VNN2, is a cell membrane protein which belongs to the CN hydrolase family and Vanin subfamily. VNN2 is widely expressed with higher expression in spleen and blood. VNN2 is a member of the vanin family of proteins which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. VNN2 is an amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine. It is involved in the thymus homing of bone marrow cells. VNN2 plays a role in transendothelial migration of neutrophils and may regulate beta-2 integrin-mediated cell adhesion, migration and motility of neutrophil.

Human VNN2/Vanin-2 References
  • Suzuki K. et al., 1999, J. Immunol. 162: 4277-84.
  • Martin, F. et al., 2001,Immunogenetics. 53 (4):296-306.
  • Liu al., 2005, J. Proteome Res. 4: 2070-80.
  • Jansen P.AM. et al., 2009, J. Invest. Dermatol. 129: 2167-74.
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