Quick Order

Human STK10 / LOK Protein (His Tag)

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
STK10Protein Product Information
Synonym:LOK, PRO2729, STK10
Protein Construction:A DNA sequence encoding the human STK10 (NP_005981.3) (Arg 18-Glu 317) was expressed, with a polyhistide tag at the N-terminus.
Species:Human
Expressed Host:E. coli
Form & Shipping:Liquid. It is shipped out with blue ice.
STK10Protein QC Testing
Purity:> 95 % as determined by SDS-PAGE
Bio-Activity:The specific activity was determined to be 1353 nmol/min/mg using synthetic AXLtide peptide (KKSRGDYMTMQIG) as substrate (see protocol for kinase assay).
Endotoxin:Please contact us for more information.
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant human STK10 consisting of 315 amino acids and has a calculated molecular mass of 36 kDa. It migrates as an approximately 40 kDa band in SDS-PAGE under reducing conditions.
Formulation:Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
STK10Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:
11724-H07E
Background

Serine / threonine-protein kinase 10, also known as Lymphocyte-oriented kinase, STK10 and LOK, which belongs to the protein kinase superfamily, STE Ser / Thr protein kinase family and STE20 subfamily. Protein kinases constitute a large superfamily of enzymes with key regulatory functions in nearly all signal transmission processes of eukaryotic cells. The Ste20 family of serine/threonine kinases plays an important role in numerous cellular functions such as growth, apoptosis, and morphogenesis. STK10 is similar to several known polo-like kinase kinases. It can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. STK10 can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway. Stk10 can associate with Plk1 in cells and furthermore can phosphorylate Plk1. It can also act on substrates such as myelin basic protein and histone 2A on serine and threonine residues.

References
  • Kuramochi S, et al., 1997, J. Biol. Chem. 272 (36): 22679-84.
  • Kuramochi S, et al., 1999, Immunogenetics. 49 (5): 369-75. 
  • Walter, SA. et al., J Biol Chem 2003, 278 (20):18221-8.
  • Related Products
    Product nameProduct name
    Size / Price
    Catalog:11724-H07E-100
    List Price: $348.00  (Save $0.00)
    Price:$348.00      [How to order]
    Availabilityon Sale
    Images
    • Human STK10 / LOK Protein (His Tag) SDS-PAGE
    Recently Viewed Items
      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"