|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat CTSD ORF mammalian expression plasmid, C-GFPSpark tag||RG81328-ACG|
|Rat CTSD ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81328-ACR|
|Rat CTSD ORF mammalian expression plasmid, C-Flag tag||RG81328-CF|
|Rat CTSD ORF mammalian expression plasmid, C-His tag||RG81328-CH|
|Rat CTSD ORF mammalian expression plasmid, C-Myc tag||RG81328-CM|
|Rat CTSD ORF mammalian expression plasmid, C-HA tag||RG81328-CY|
|Rat CTSD ORF mammalian expression plasmid, N-Flag tag||RG81328-NF|
|Rat CTSD ORF mammalian expression plasmid, N-His tag||RG81328-NH|
|Rat CTSD ORF mammalian expression plasmid, N-Myc tag||RG81328-NM|
|Rat CTSD ORF mammalian expression plasmid, N-HA tag||RG81328-NY|
|Rat CTSD Gene cDNA clone plasmid||RG81328-U|
|Rat CTSD natural ORF mammalian expression plasmid||RG81328-UT|
|Learn more about expression Vectors|
Cathepsin D (CTSD), a well known lysosomal aspartyl protease and belongs to the peptidase C1 family, which is a normal and major component of lysosomes, and is found in almost all cells and tissues of mammals. Its mostly described function is intracellular catabolism in lysosomal compartments, other physiological effect include hormone and antigen processing. Cathepsin D has a specificity similar to but narrower than that of pepsin A. Cathepsin D plays an important role in the degradation of proteins, the generation of bioactive proteins, antigen processing, etc. Among different role in cell physiology, a new function of this enzyme is examined. Cathepsin D is an important regulator of apoptotic pathways in cells. It acts at different stage of intrinsic and extrinsic pathway of apoptosis. In addition, CTSD secreted from human prostate carcinoma cells are responsible for the generation of angiostatin, a potent endogenous inhibitor of angiogenesis, suggesting its contribution to the prevention of tumor growth and angiogenesis-dependent growth of metastases.