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Influenza A H1N1 Hemagglutinin / HA Antibody, Mouse MAb

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HAAntibody Product Information
Immunogen:Recombinant H1N1 (A/Puerto Rico/8/1934) HA protein (Catalog#11684-V08H)
Clone ID:2F1A7
Ig Type:Mouse IgG1
Concentration:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant H1N1 (A/Puerto Rico/8/1934) HA (Catalog#11684-V08H; ABD77675.1; Met 1-Gln 528). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
HAAntibody Usage Guide
Specificity:H1N1 (A/Puerto Rico/8/1934) HA
No cross-reactivity in ELISA with
H1N1 (A/California/07/2009) HA
H1N1 (A/California/04/2009) HA
H1N1 (A/California/04/2009) HA1
H1N1 (A/Brisbane/59/2007) HA
H1N1 (A/Brisbane/59/2007) HA1
H1N1 (A/BrevigMission/1/1918) HA
H1N1 (A/Solomon Islands/3/2006)
H1N1 (A/Ohio/UR06-0091/2007) HA
H1N1 (A/New Caledonia/20/1999) HA
H1N1 (A/WSN/1933) HA
H1N2 (A/swine/Guangxi/13/2006) HA
H1N3 (A/duck/NZL/160/1976)
H5N1 (A/Anhui/1/2005) HA
H5N1 (A/Anhui/1/2005) HA2
Application:WB, ELISA

WB: 1-2 μg/mL

This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect H1N1 (A/Puerto Rico/8/1934) HA in WB. Using a DAB detection system, the detection limit for H1N1 (A/Puerto Rico/8/1934) HA is approximately 8 ng/lane under non-reducing conditions.Use of this antibody under reducing conditions is not recommended.

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect H1N1 (A/Puerto Rico/8/1934) HA. The detection limit for H1N1 (A/Puerto Rico/8/1934) HA is approximately 2.5 ng/well.

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"