|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat CFL2 ORF mammalian expression plasmid, C-GFPSpark tag||RG81236-ACG|
|Rat CFL2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81236-ACR|
|Rat CFL2 ORF mammalian expression plasmid, N-GFPSpark tag||RG81236-ANG|
|Rat CFL2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG81236-ANR|
|Rat CFL2 ORF mammalian expression plasmid, C-Flag tag||RG81236-CF|
|Rat CFL2 ORF mammalian expression plasmid, C-His tag||RG81236-CH|
|Rat CFL2 ORF mammalian expression plasmid, C-Myc tag||RG81236-CM|
|Rat CFL2 ORF mammalian expression plasmid, C-HA tag||RG81236-CY|
|Rat CFL2 ORF mammalian expression plasmid, N-Flag tag||RG81236-NF|
|Rat CFL2 ORF mammalian expression plasmid, N-His tag||RG81236-NH|
|Rat CFL2 ORF mammalian expression plasmid, N-Myc tag||RG81236-NM|
|Rat CFL2 ORF mammalian expression plasmid, N-HA tag||RG81236-NY|
|Rat CFL2 Gene cDNA clone plasmid||RG81236-U|
|Rat CFL2 natural ORF mammalian expression plasmid||RG81236-UT|
|Learn more about expression Vectors|
Cofilin 2 (muscle), also known as CFL2, is a member of cofilin family of the actin-binding protein superfamily. Cofilin2 shows significant homology to the other two members: cofilin 1 and DSTN, through its entire sequence, and contains residues conserved among the cofilin family that are responsible for actin-binding. Cofilin 2 (CFL2) is an important regulator of striated myocyte function. Purified cofilin 2 depolymerized actin filaments in a dose- and pH-dependent manner and reduced the apparent viscosity of an actin solution, although they did not co-sediment with actin filaments at all. Cofilin2 is not expressed in vegetative cells, but is transiently induced during the aggregation stage of development, whereas cofilin 1 was predominantly expressed in vegetative cells.