|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse PARP1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50753-ACG|
|Mouse PARP1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50753-ACR|
|Mouse PARP1 ORF mammalian expression plasmid, N-GFPSpark tag||MG50753-ANG|
|Mouse PARP1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50753-ANR|
|Mouse PARP1 ORF mammalian expression plasmid, C-Flag tag||MG50753-CF|
|Mouse PARP1 ORF mammalian expression plasmid, C-His tag||MG50753-CH|
|Mouse PARP1 ORF mammalian expression plasmid, C-Myc tag||MG50753-CM|
|Mouse PARP1 ORF mammalian expression plasmid, C-HA tag||MG50753-CY|
|Mouse PARP1 Gene cDNA clone plasmid||MG50753-G|
|Mouse PARP1 ORF mammalian expression plasmid, N-Flag tag||MG50753-NF|
|Mouse PARP1 ORF mammalian expression plasmid, N-His tag||MG50753-NH|
|Mouse PARP1 ORF mammalian expression plasmid, N-Myc tag||MG50753-NM|
|Mouse PARP1 ORF mammalian expression plasmid, N-HA tag||MG50753-NY|
|Mouse PARP1 natural ORF mammalian expression plasmid||MG50753-UT|
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Poly (ADP-ribose) polymerase 1(PRAP1), also known as NAD(+) ADP-ribosyltransferase 1(ADPRT), is a chromatin-associated enzyme which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. The poly(ADP-ribosyl)ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis. PARP1 is demonstrateed to mediate the poly(ADP-ribose) ation of APLF (aprataxin PNK-like factor) and CHFR (checkpoint protein with FHA and RING domains), two representative proteins involved in the DNA damage response and checkpoint regulation. Further, It has been suggested that DNA-dependent protein kinase (DNA-PK), another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes. PARP1 inhibitors is thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.