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Human LRP1 / A2MR / CD91 (aa 786-1165) HEK293 Cell Lysate (WB positive control)

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Human LRP1 Transfected / Overexpression Cell Lysate Product Information
Expressed Host:Human Cells
Product Description:Human Cell lysate that Human LRP1 / A2MR / CD91 (aa 786-1165) transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
Sequence information:The amino acid sequence (Arg 786-Leu 1165) of human LRP1 (NP_002323.2) corresponding to the cluster II LDLR domain was expressed and purified, with an additional peptide (ALEVLFQ) at the C-terminus.
Predicted N Terminal:Glu 27
Molecule Mass:The recombinant human LRP1 (aa 786-1165) consists of 387 amino acids and predicts a molecular mass of 42 kDa. As a result of glycosylation, the apparent molecular mass of the protein is approximately 90-120 kDa in SDS-PAGE under reducing conditions.
Human LRP1 Transfected / Overexpression Cell Lysate Usage Guide
Preparation Method:Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer:Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing:12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
Stability:Samples are stable for up to twelve months from date of receipt.
Recommend Usage:1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
Storage Buffer:1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Storage Instruction:Store at 4℃. After re-dissolution, aliquot and store at -80℃.
Application notes:Western blot (WB): Use at an assay dependent dilution.
Other Applications: Not tested.
Optimal dilutions/concentrations should be determined by the end user.
LRP1 (CD91) Background

Low density lipoprotein receptor-related protein 1 (LRP1), also known as alpha-2-macroglobulin receptor (A2MR), apolipoprotein E receptor (APOER) or cluster of differentiation 91 (CD91), is an endocytic receptor involved in several cellular processes. LRP1 / CD91 is a member of the cluster of differentiation (CD) system. The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. LRP1 / CD91 is involved in endocytosis and in phagocytosis of apoptotic cells and is required for early embryonic development. LRP1 / CD91 is also Involved in cellular lipid homeostasis, the plasma clearance of chylomicron remnants, activated LRPAP1 (alpha 2-macroglobulin), and the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. LRP1 / CD91 May modulate cellular events, such as APP metabolism kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission need the involvement of LRP1 / CD91.

Human LRP1 (CD91) References
  • Su HP, et al. (2002) Interaction of CED-6 / GULP, an adapter protein involved in engulfment of apoptotic cells with CED-1 and CD91 / low density lipoprotein receptor-related protein (LRP). J Biol Chem. 277(14): 11772-9.
  • Chen JS, et al. (2010) Secreted heat shock protein 90alpha induces colorectal cancer cell invasion through CD91 / LRP-1 and NF-kappaB-mediated integrin alphaV expression. J Biol Chem. 285(33): 25458-66.
  • Dasgupta S, et al. (2008) Factor VIII bypasses CD91 / LRP for endocytosis by dendritic cells leading to T-cell activation. Haematologica. 93(1): 83-9.
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    Catalog: 11665-HCCHL-300
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