|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse CD59A ORF mammalian expression plasmid, C-GFPSpark tag||MG50724-ACG|
|Mouse CD59A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50724-ACR|
|Mouse CD59A ORF mammalian expression plasmid, C-Flag tag||MG50724-CF|
|Mouse CD59A ORF mammalian expression plasmid, C-His tag||MG50724-CH|
|Mouse CD59A ORF mammalian expression plasmid, C-Myc tag||MG50724-CM|
|Mouse CD59A ORF mammalian expression plasmid, C-HA tag||MG50724-CY|
|Mouse CD59A Gene cDNA clone plasmid||MG50724-G|
|Mouse CD59A ORF mammalian expression plasmid, N-Flag tag||MG50724-NF|
|Mouse CD59A ORF mammalian expression plasmid, N-His tag||MG50724-NH|
|Mouse CD59A ORF mammalian expression plasmid, N-Myc tag||MG50724-NM|
|Mouse CD59A ORF mammalian expression plasmid, N-HA tag||MG50724-NY|
|Mouse CD59A natural ORF mammalian expression plasmid||MG50724-UT|
|Learn more about expression Vectors|
Protectin, a complement regulatory protein, also known as CD59, or MIRL (membrane inhibitor of reactive lysis) is a small protein that inhibits the complement membrane attack complex by binding C5b678 and preventing C9 from binding and polymerizing. The amino-terminal 25 amino acids represented a typical signal peptide sequence and the carboxy-terminal hydrophobic amino acids were characteristic for phosphatidylinositol-anchored proteins.It was found that the CD59/Protectin antigen is a small protein sometimes associated with larger components (45 and 80 kD) in urine. CD59/Protectin antigen was released from the surface of transfected COS cells by phosphatidylinositol-specific phospholipase C, demonstrating that it is attached to the cell membrane by means of a glycolipid anchor; it is therefore likely to be absent from the surface of affected erythrocytes in the disease paroxysmal nocturnal hemoglobinuria.