|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat NCSTN ORF mammalian expression plasmid, C-GFPSpark tag||RG81043-ACG|
|Rat NCSTN ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81043-ACR|
|Rat NCSTN ORF mammalian expression plasmid, C-Flag tag||RG81043-CF|
|Rat NCSTN ORF mammalian expression plasmid, C-His tag||RG81043-CH|
|Rat NCSTN ORF mammalian expression plasmid, C-Myc tag||RG81043-CM|
|Rat NCSTN ORF mammalian expression plasmid, C-HA tag||RG81043-CY|
|Rat NCSTN ORF mammalian expression plasmid, N-Flag tag||RG81043-NF|
|Rat NCSTN ORF mammalian expression plasmid, N-His tag||RG81043-NH|
|Rat NCSTN ORF mammalian expression plasmid, N-Myc tag||RG81043-NM|
|Rat NCSTN ORF mammalian expression plasmid, N-HA tag||RG81043-NY|
|Rat NCSTN Gene cDNA clone plasmid||RG81043-U|
|Rat NCSTN natural ORF mammalian expression plasmid||RG81043-UT|
|Learn more about expression Vectors|
Nicastrin (NCST, or NCT), a single-pass membrane glycoprotein that harbors a large extracellular domain, is an essential component of the gamma-secretase complex. Several lines of evidence indicate that the members of these complexes could also contribute to the control of cell death. NCT controls cell death via phosphoinositide 3-kinase/Akt and p53-dependent pathways and that this function remains independent of the activity and molecular integrity of the gamma-secretase complexes. Increasing evidences have shown that Nicastrin/NCSTN plays a crucial role in gamma-cleavage of the amyloid precursor protein (APP). The glycoprotein Nicastrin is an essential component of the gamma-secretase complex, a high molecular weight complex which also contains the presenilin proteins, Aph-1 and Pen-2. The gamma-secretase complex is not only involved in APP processing but also in the processing of an increasing number of other type I integral membrane proteins. As the largest subunit of the gamma-secretase complex, Nicastrin plays a crucial role in its activation. Inhibition of NCSTN demonstrated an altered gamma-cleavage activity, suggesting its potential implication in developing Alzheimer's disease (AD). In addition, Nicastrin can function to maintain epithelial to mesenchymal transition during breast cancer progression. Anti-nicastrin polyclonal and monoclonal antibodies were able to decrease notch1 and vimentin expression and reduced the invasive capacity of breast cancer cells in vitro.