|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse RSPO3 ORF mammalian expression plasmid, C-GFPSpark tag||MG50606-ACG|
|Mouse RSPO3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50606-ACR|
|Mouse RSPO3 ORF mammalian expression plasmid, C-Flag tag||MG50606-CF|
|Mouse RSPO3 ORF mammalian expression plasmid, C-His tag||MG50606-CH|
|Mouse RSPO3 ORF mammalian expression plasmid, C-Myc tag||MG50606-CM|
|Mouse RSPO3 ORF mammalian expression plasmid, C-HA tag||MG50606-CY|
|Mouse RSPO3 Gene cDNA clone plasmid||MG50606-M|
|Mouse RSPO3 ORF mammalian expression plasmid, N-Flag tag||MG50606-NF|
|Mouse RSPO3 ORF mammalian expression plasmid, N-His tag||MG50606-NH|
|Mouse RSPO3 ORF mammalian expression plasmid, N-Myc tag||MG50606-NM|
|Mouse RSPO3 ORF mammalian expression plasmid, N-HA tag||MG50606-NY|
|Mouse RSPO3 natural ORF mammalian expression plasmid||MG50606-UT|
|Learn more about expression Vectors|
R-spondin 3 (RSPO3) is a member of the R-Spondin (RSPO) family in vertebrates that activate Wnt/beta-catenin signaling, plays a key role in these processes. The RSPO family of secreted Wnt modulators is involved in development and disease and holds therapeutic promise as stem cell growth factors. The four members have high structural homology. RSPO2 and RSPO3 are more potent than RSPO1, whereas RSPO4 is relatively inactive. All RSPO members require Wnt ligands and LRP6 for activity and amplify signaling of Wnt3A, Wnt1, and Wnt7A, suggesting that RSPO proteins are general regulators of canonical Wnt signaling. RSPO3/PCP signaling during gastrulation requires Wnt5a and is transduced via Fz7, Dvl, and JNK. RSPO3 functions by inducing Sdc4-dependent, clathrin-mediated endocytosis. RSPO3 is a novel, evolutionarily conserved angiogenic factor in embryogenesis. RSPO3 has a key role in the interaction between chorion and allantois in labyrinthine development.