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Human CSNK1G1 / CKI-gamma 1 Protein (His & GST Tag)

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CSNK1G1Protein Product Information
Synonym:CKI-gamma 1, casein kinase I isoform gamma-1, CSNK1G1
Protein Construction:A DNA sequence encoding full length of human CSNK1G1 (NP_071331.2) (Met 1-Lys 422) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Expressed Host:Baculovirus-Insect Cells
Form & Shipping:Liquid. It is shipped out with blue ice.
CSNK1G1Protein QC Testing
Purity:> 94 % as determined by SDS-PAGE
Bio-Activity:The specific activity was determined to be 4 nmol/min/mg using casein as substrate (see protocol for kinase assay).
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant human CSNK1G1/GST chimera consists of 659 amino acids and has a calculated molecular mass of 76.3 kDa. It migrates as an approximately 66 kDa band in SDS-PAGE under reducing conditions.
Formulation:Supplied as sterile 20mM Tris, 500mM NaCl, 10% gly, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
CSNK1G1Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Casein kinase I isoform gamma-1, also known as CSNK1G1, is a member of the protein kinase superfamily, CK1 Ser/Thr protein kinase family and casein kinase I subfamily. The casein kinase I family of protein kinases are serine / threonine-selective enzymes that function as regulators of signal transduction pathways in most eukaryotic cell types. Casein has been used as a substrate since the earliest days of research on protein phosphorylation. Casein kinase activity associated with the endoplasmic reticulum of mammary glands was first characterized in 1974 and its activity was shown to not depend on cyclic AMP. The CKI family of monomeric serine–threonine protein kinases is found in eukaryotic organisms from yeast to human. Mammals have seven family members: alpha, beta 1, gamma 1, gamma 2, gamma 3, delta, and epsilon. The family members have the highest homology in their kinase domains (53%–98% identical) and differ from most other protein kinases by the presence of the sequence S-I-N instead of A-P-E in kinase domain VIII. The CKI family members appear to have similar substrate specificity and substrate selection is thought to be regulated via subcellular localization and docking sites in specific substrates.

  • E. Bingham. et al.,1974, Journal of Biological Chemistry. 249: 3647-51.
  • Wojciech S. et al., 2004,  Journal of Biological Chemistry.279: 13011-7.
  • L. Lum. et al., 2004, Science Volume 304, pages 1755-9.
  • R. Takada. et al., 2005, Genes Cells Volume 10: 919-28.
  • G. Davidson. et al., 2005, Nature Volume 438:  867-72.
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    • Human CSNK1G1 / CKI-gamma 1 Protein (His & GST Tag) SDS-PAGE
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