|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat S100A6 ORF mammalian expression plasmid, C-GFPSpark tag||RG80826-ACG|
|Rat S100A6 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80826-ACR|
|Rat S100A6 ORF mammalian expression plasmid, N-GFPSpark tag||RG80826-ANG|
|Rat S100A6 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80826-ANR|
|Rat S100A6 ORF mammalian expression plasmid, C-Flag tag||RG80826-CF|
|Rat S100A6 ORF mammalian expression plasmid, C-His tag||RG80826-CH|
|Rat S100A6 ORF mammalian expression plasmid, C-Myc tag||RG80826-CM|
|Rat S100A6 ORF mammalian expression plasmid, C-HA tag||RG80826-CY|
|Rat S100A6 ORF mammalian expression plasmid, N-Flag tag||RG80826-NF|
|Rat S100A6 ORF mammalian expression plasmid, N-His tag||RG80826-NH|
|Rat S100A6 ORF mammalian expression plasmid, N-Myc tag||RG80826-NM|
|Rat S100A6 ORF mammalian expression plasmid, N-HA tag||RG80826-NY|
|Rat S100A6 Gene cDNA clone plasmid||RG80826-U|
|Rat S100A6 natural ORF mammalian expression plasmid||RG80826-UT|
|Learn more about expression Vectors|
S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. S100A6 (S100 calcium binding protein A6) is a member of the S100 family of proteins, and functions in prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of S100A6 have been implicated in melanoma.