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ARG1 / Arginase 1 Antibody, Rabbit MAb

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Human ARG1 Antibody Product Information
Immunogen:Recombinant Human ARG1 / Arginase 1 protein (Catalog#11558-H08H)
Clone ID:034
Ig Type:Rabbit IgG
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant Human ARG1 / Arginase 1 (rh ARG1 / Arginase 1; Catalog#11558-H08H; P05089-1; Met 1-Lys 322).
Human ARG1 Antibody Usage Guide
Specificity:Human ARG1 / Arginase 1
Application:WB, ELISA, IHC-P, IP

WB: 5-10 μg/mL

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human ARG1. The detection limit for Human ARG1 is approximately 0.00975 ng/well.

IHC-P: 1-10 μg/mL

IP: 2-6 μg/mg of lysate

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human ARG1 Antibody WB Application Image
ARG1 / Arginase 1 Antibody, Rabbit MAb, Western blot
Human ARG1 Antibody IHC Application Image
[Click to enlarge image]
Caption:
Immunochemical staining of human ARG1 in human kidney with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).
ARG1 / Arginase 1 Antibody, Rabbit MAb, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human ARG1 in human liver with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).
ARG1 / Arginase 1 Antibody, Rabbit MAb, Immunohistochemistry
Human ARG1 Antibody IP Application Image
ARG1 / Arginase 1 Antibody, Rabbit MAb, Immunoprecipitation
Other ARG1 Antibody Products
ARG1/Arginase 1 Background

Arginase is the focal enzyme of the urea cycle hydrolysing L-arginine to urea and L-ornithine. Emerging studies have identified arginase in the vasculature and have implicated this enzyme in the regulation of nitric oxide (NO) synthesis and the development of vascular disease. Arginase also redirects the metabolism of L-arginine to L-ornithine and the formation of polyamines and L-proline, which are essential for smooth muscle cell growth and collagen synthesis. Arginase is encoded by two recently discovered genes (Arginase I and Arginase II). In most mammals, Arginase 1 (ARG1) also known as Arginase, liver, which functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. It is located in mitochondria of several tissues in the body, with most abundance in the kidney and prostate. It may be found at lower levels in macrophages, lactating mammary glands, and brain.

Human ARG1/Arginase 1 References
  • Durante W, et al. (2007) Arginase: a critical regulator of nitric oxide synthesis and vascular function. Clin Exp Pharmacol Physiol. 34(9): 906-11.
  • Waddington SN. (2002) Arginase in glomerulonephritis. Kidney Int. 61(3): 876-81.
  • Morris SM. (2002). Regulation of enzymes of the urea cycle and arginine metabolism. Annual review of nutrition. 22 (1): 87-105.
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