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Human DOT1L / KMT4 Protein

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Human DOT1L Protein Product Information
Synonym:DKFZp586P1823, DOT1, KIAA1814, KMT4
Protein Construction:A DNA sequence encoding the human DOT1L (NP_115871.1) N-terminal segment (Gly 2-Lys 416) was expressed and purified, with two additional amino acids (Gly & Pro) at the N-terminus.
Expressed Host:E. coli
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human DOT1L Protein QC Testing
Purity:> 90 % as determined by SDS-PAGE
Endotoxin:Please contact us for more information.
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Gly
Molecule Mass:The recombinant human DOT1L comprises 417 amino acids and has a predicted molecular mass of 47.6 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions as predicted.
Formulation:Lyophilized from sterile 20mM HEPES, 150mM NaCl, 1mM EDTA, 15% glycerol, pH 7.5
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human DOT1L Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human DOT1L Protein SDS-PAGE
Human DOT1L / KMT4 Protein SDS-PAGE
Other DOT1L Recombinant Protein Products
DOT1L/KMT4 Background

Histone-lysine N-methyltransferase, H3 lysine-79 specific, also known as Histone H3-K79 methyltransferase, DOT1-like protein, Lysine N-methyltransferase 4 and DOT1L, is a nucleus protein which belongs to the DOT1 family. In contrast to other lysine histone methyltransferase, DOT1L does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones. DOT1L is an histone methyltransferase. It methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. DOT1L binds to DNA. Methylation of lysine 79 on histone H3 (H3K79) is mediated by DOT1L. It is involved in the regulation of telomeric silencing, development, cell cycle checkpoint and transcription. Mass spectrometry of the DOT1L-containing complex revealed that AF9, ENL and NPM1 were shown to be major DOT1L-interacting proteins. DOT1L might control AF9- and ENL-mediated transcription, regulate RNA processing, and function as a histone chaperone in a NPM1-dependent manner.

Human DOT1L/KMT4 References
  • Nagase T. et al., 2001, DNA Res. 8:85-95.
  • Feng Q.et al., 2002, Curr. Biol. 12:1052-8.
  • Dephoure N. et al., 2008, Proc. Natl. Acad. Sci. USA. 105:10762-7.
  • Gauci S. et al., 2009, Anal. Chem. 81:4493-501.
  • Park,G. et al.,  2010, Protein J. 29 (3):213-23. 
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    Catalog: 11552-HNCE-20
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    • Human DOT1L / KMT4 Protein SDS-PAGE
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