After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!
Text Size:AAA

Human TLK2 / PKU-ALPHA Protein (aa 397-772)

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
Human TLK2 Protein Product Information
Protein Construction:A DNA sequence encoding the human TLK2 (Q86UE8-1) (Leu397-Asn772) was fused with two additional amino acids (Gly & Pro) at the N-terminus.
Expressed Host:Baculovirus-Insect Cells
Shipping:Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Human TLK2 Protein QC Testing
Purity:> 95 % as determined by SDS-PAGE
Bio-Activity:Kinase activity untested
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Gly
Molecule Mass:The recombinant human TLK2 consists of 378 amino acids and has a calculated molecular mass of 43.6 kDa. The recombinant protein migrates as an approximately 44 kDa band in SDS-PAGE under reducing conditions.
Formulation:Supplied as sterile 20mM Tris, 500mM NaCl, 3mM DTT, 10% glycerol, pH 8.0.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human TLK2 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human TLK2 Protein SDS-PAGE
Human TLK2 / PKU-ALPHA Protein (aa 397-772) SDS-PAGE
Human TLK2 / PKU-ALPHA Protein (aa 397-772) SDS-PAGE
Other TLK2 Recombinant Protein Products
TLK2/PKU-ALPHA Background

Serine / threonine-protein kinase tousled-like 2, also known as PKU-alpha, Tousled-like kinase 2 and TLK2, is a nucleus protein which belongs to the protein kinase superfamily and Ser/Thr protein kinase family. The tousled-like kinases are an evolutionarily conserved family of proteins implicated in DNA repair, DNA replication and mitosis in metazoans and plants. Their absence from the yeasts and other eukaryotic 'microbes' suggests a specific role for them in the development of multicellular organisms. Tousled-like kinase 2 / TLK2 is widely expressed. It is present in fetal placenta, liver, kidney, pancreas, heart and skeletal muscle. It is also found in adult cell lines. Tousled-like kinase 2 / TLK2 contains one protein kinase domain. Tousled-like kinase 2 / TLK2 is rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly.

Human TLK2/PKU-ALPHA References
  • Sillje HHW. et al., 2001,Curr Biol. 11: 1068-73.
  • Groth A. et al., 2003, EMBO J. 22: 1676-87.
  • Beausoleil SA. et al., 2004, Proc Natl Acad Sci. 101: 12130-5.
  • Mayya V. et al., 2009, Sci Signal. 2: RA46.
  • Oppermann FS. et al., 2009, Mol Cell Proteomics. 8: 1751-64.
  • Product nameProduct name
    Size / Price
    Catalog: 11551-HNCB-50
    List Price:   (Save )
    Price:      [How to order]
    AvailabilityIn StockShipping instructions