|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat PPIA ORF mammalian expression plasmid, C-GFPSpark tag||RG80767-ACG|
|Rat PPIA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80767-ACR|
|Rat PPIA ORF mammalian expression plasmid, N-GFPSpark tag||RG80767-ANG|
|Rat PPIA ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80767-ANR|
|Rat PPIA ORF mammalian expression plasmid, C-Flag tag||RG80767-CF|
|Rat PPIA ORF mammalian expression plasmid, C-His tag||RG80767-CH|
|Rat PPIA ORF mammalian expression plasmid, C-Myc tag||RG80767-CM|
|Rat PPIA ORF mammalian expression plasmid, C-HA tag||RG80767-CY|
|Rat PPIA ORF mammalian expression plasmid, N-Flag tag||RG80767-NF|
|Rat PPIA ORF mammalian expression plasmid, N-His tag||RG80767-NH|
|Rat PPIA ORF mammalian expression plasmid, N-Myc tag||RG80767-NM|
|Rat PPIA ORF mammalian expression plasmid, N-HA tag||RG80767-NY|
|Rat PPIA Gene cDNA clone plasmid||RG80767-U|
|Rat PPIA natural ORF mammalian expression plasmid||RG80767-UT|
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Mouse peptidyl-prolyl cis-trans isomerase A, also known as PPIase A, Rotamase A, Cyclophilin A, Cyclosporin A-binding protein, PPIA and CYPA, is a cytoplasm protein which belongs to the cyclophilin-type PPIase family and PPIase A subfamily. Cyclophilins (CyPs) are a family of proteins found in organisms ranging from prokaryotes to humans. These molecules exhibit peptidyl-prolyl isomerase activity, suggesting that they influence the conformation of proteins in cells. PPIA / Cyclophilin A accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIA / Cyclophilin A is secreted by vascular smooth muscle cells in response to inflammatory stimuli, and could thus contribute to atherosclerosis. It is not essential for mammalian cell viability. PPIA / Cyclophilin A can interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions.