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Human TAOK3 / JIK / MAP3K18 Protein (aa 1-411, His & GST Tag)

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Human TAOK3 Protein Product Information
Synonym:DKFZp666H245, DPK, FLJ31808, JIK, MAP3K18
Protein Construction:A DNA sequence encoding the amino acids (Met 1-Asp 411) of human TAOK3 (Q9H2K8-1) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Expressed Host:Baculovirus-Insect Cells
Shipping:Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Human TAOK3 Protein QC Testing
Purity:> 92 % as determined by SDS-PAGE
Bio-Activity:The specific activity was determined to be 10 nmol/min/mg using synthetic PKCtide peptide (ERMRPRKRQGSVRRRV) as substrate.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant human TAOK3 (aa 1-411)/GST chimera consists of 648 amino acids and has a calculated molecular mass of 74.3 kDa. It migrates as an approximately 85 kDa band in SDS-PAGE under reducing conditions.
Formulation:Supplied as sterile 20mM Tris, 500mM NaCl, 3mM GSH, pH 7.4, 10% gly
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human TAOK3 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human TAOK3 Protein SDS-PAGE
Human TAOK3 / JIK / MAP3K18 Protein (aa 1-411, His & GST Tag) SDS-PAGE
Human TAOK3 / JIK / MAP3K18 Protein (aa 1-411, His & GST Tag) SDS-PAGE
Other TAOK3 Recombinant Protein Products
TAOK3/JIK Background

Serine/threonine-protein kinase TAO3, also known as cutaneous T-cell lymphoma-associated antigen HD-CL-09, CTCL-associated antigen HD-CL-09, Dendritic cell-derived protein kinase, JNK / SAPK-inhibitory kinase, Jun kinase-inhibitory kinase, Kinase from chicken homolog A, Thousand and one amino acid protein 3, JIK, TAOK3 and MAP3K18, is cytoplasm and peripheral membrane protein which belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. Protein kinases are pivotal regulators of cell signaling that modulate each other's functions and activities through site-specific phosphorylation events. TAOK3 / JIK contains one protein kinase domain. TAOK3 / JIK is ubiquitously expressed at a low level, and highly expressed in peripheral blood leukocytes (PBLs), thymus, spleen, kidney, skeletal muscle, heart and liver. TAOK3 / JIK inhibits the basal activity of Jun kinase. It is negatively regulated by epidermal growth factor (EGF). When overexpressed, TAOK3 / JIK may activate ERK1 / ERK2 and JNK / SAPK.

Human TAOK3/JIK References
  • Tassi E., et al., 1999, J. Biol. Chem. 274:33287-95.
  • Yoneda T., et al., 2001, J. Biol. Chem. 276:13935-40.
  • Yustein J.T., et al.,2003, Oncogene 22:6129-41.
  • Daub H., et al., 2008, Mol. Cell 31:438-48.
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    Catalog: 11547-H20B-50
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