|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat IGF1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80503-ACG|
|Rat IGF1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80503-ACR|
|Rat IGF1 ORF mammalian expression plasmid, C-Flag tag||RG80503-CF|
|Rat IGF1 ORF mammalian expression plasmid, C-His tag||RG80503-CH|
|Rat IGF1 ORF mammalian expression plasmid, C-Myc tag||RG80503-CM|
|Rat IGF1 ORF mammalian expression plasmid, C-HA tag||RG80503-CY|
|Rat IGF1 ORF mammalian expression plasmid, N-Flag tag||RG80503-NF|
|Rat IGF1 ORF mammalian expression plasmid, N-His tag||RG80503-NH|
|Rat IGF1 ORF mammalian expression plasmid, N-Myc tag||RG80503-NM|
|Rat IGF1 ORF mammalian expression plasmid, N-HA tag||RG80503-NY|
|Rat IGF1 Gene cDNA clone plasmid||RG80503-U|
|Rat IGF1 natural ORF mammalian expression plasmid||RG80503-UT|
|Learn more about expression Vectors|
IGF I, also known as mechano growth factor, somatomedin-C, IGF-I and IGF1, is a secreted protein which belongs to the?insulin family. The insulin family, comprised of insulin, relaxin, insulin-like growth factors I and II ( IGF-I and IGF-II ) and possibly the beta-subunit of 7S nerve growth factor, represents a group of structurally related polypeptides whose biological functions have diverged. The IGFs, or somatomedins, constitute a class of polypeptides that have a key role in pre-adolescent mammalian growth. IGF-I expression is regulated by GH and mediates postnatal growth, while IGF-II appears to be induced by placental lactogen during prenatal development. IGF1 / IGF-I may be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. IGF1 / IGF-I stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. Defects in IGF1 / IGF-I are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.