|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat FTL ORF mammalian expression plasmid, C-GFPSpark tag||RG80491-ACG|
|Rat FTL ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80491-ACR|
|Rat FTL ORF mammalian expression plasmid, N-GFPSpark tag||RG80491-ANG|
|Rat FTL ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80491-ANR|
|Rat FTL ORF mammalian expression plasmid, C-Flag tag||RG80491-CF|
|Rat FTL ORF mammalian expression plasmid, C-His tag||RG80491-CH|
|Rat FTL ORF mammalian expression plasmid, C-Myc tag||RG80491-CM|
|Rat FTL ORF mammalian expression plasmid, C-HA tag||RG80491-CY|
|Rat FTL ORF mammalian expression plasmid, N-Flag tag||RG80491-NF|
|Rat FTL ORF mammalian expression plasmid, N-His tag||RG80491-NH|
|Rat FTL ORF mammalian expression plasmid, N-Myc tag||RG80491-NM|
|Rat FTL ORF mammalian expression plasmid, N-HA tag||RG80491-NY|
|Rat FTL Gene cDNA clone plasmid||RG80491-U|
|Rat FTL natural ORF mammalian expression plasmid||RG80491-UT|
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Ferritin, light polypeptide (FTL) is the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Storage of iron in the tissues occurs in the form of ferritin and hemosiderin. The latter originates from ferritin that has undergone intracellular digestion of its protein shell, leaving the iron core. Ferritin and hemosiderin are components of a continuum. Ferritin has been identified in all types of living organisms: animals, plants, molds, and bacteria. Whithin the protein shell of ferritin, iron is first oxidized to the ferric state for storage as ferric oxyhdroxide. Thus, ferritin removes excess iron from the cell sap where it could otherwise participate in peroxidation mechanisms.