|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat NGF ORF mammalian expression plasmid, C-GFPSpark tag||RG80426-ACG|
|Rat NGF ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80426-ACR|
|Rat NGF ORF mammalian expression plasmid, C-Flag tag||RG80426-CF|
|Rat NGF ORF mammalian expression plasmid, C-His tag||RG80426-CH|
|Rat NGF ORF mammalian expression plasmid, C-Myc tag||RG80426-CM|
|Rat NGF ORF mammalian expression plasmid, C-HA tag||RG80426-CY|
|Rat NGF Gene cDNA clone plasmid||RG80426-G|
|Rat NGF ORF mammalian expression plasmid, His tag||RG80426-G-H|
|Rat NGF ORF mammalian expression plasmid, N-Flag tag||RG80426-NF|
|Rat NGF ORF mammalian expression plasmid, N-His tag||RG80426-NH|
|Rat NGF ORF mammalian expression plasmid, N-Myc tag||RG80426-NM|
|Rat NGF ORF mammalian expression plasmid, N-HA tag||RG80426-NY|
|Rat NGF natural ORF mammalian expression plasmid||RG80426-UT|
|Learn more about expression Vectors|
Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. In addition, previous studies indicated that NGF may also have an important role in the regulation of the immune system.