|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat IL20 ORF mammalian expression plasmid, C-GFPSpark tag||RG80422-ACG|
|Rat IL20 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80422-ACR|
|Rat IL20 ORF mammalian expression plasmid, C-Flag tag||RG80422-CF|
|Rat IL20 ORF mammalian expression plasmid, C-His tag||RG80422-CH|
|Rat IL20 ORF mammalian expression plasmid, C-Myc tag||RG80422-CM|
|Rat IL20 ORF mammalian expression plasmid, C-HA tag||RG80422-CY|
|大鼠 IL20 基因全长ORF克隆||RG80422-G|
|Rat IL20 ORF mammalian expression plasmid, N-Flag tag||RG80422-NF|
|Rat IL20 ORF mammalian expression plasmid, N-His tag||RG80422-NH|
|Rat IL20 ORF mammalian expression plasmid, N-Myc tag||RG80422-NM|
|Rat IL20 ORF mammalian expression plasmid, N-HA tag||RG80422-NY|
|Rat IL20 natural ORF mammalian expression plasmid||RG80422-UT|
|Learn more about expression Vectors|
IL20/Interleukin-20 belongs to the IL-10 family. It is a cytokine structurally related to interleukin 10. IL20/Interleukin-20 can be detected in skin, trachea, and other tissues. It is produced by activated keratinocytes and monocytes and transmits an intracellular signal through two distinct cell-surface receptor complexes on keratinocytes and other epithelial cells.It has been shown that interleukin-20 transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. It may be involved in epidermal function and psoriasis. It also regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. In addition, IL20/Interleukin-20 also causes cell expansion of multipotential hematopoietic progenitor cells. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis.