|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat CNTN2 ORF mammalian expression plasmid, C-GFPSpark tag||RG80420-ACG|
|Rat CNTN2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80420-ACR|
|Rat CNTN2 ORF mammalian expression plasmid, C-Flag tag||RG80420-CF|
|Rat CNTN2 ORF mammalian expression plasmid, C-His tag||RG80420-CH|
|Rat CNTN2 ORF mammalian expression plasmid, C-Myc tag||RG80420-CM|
|Rat CNTN2 ORF mammalian expression plasmid, C-HA tag||RG80420-CY|
|Rat CNTN2 Gene cDNA clone plasmid||RG80420-G|
|Rat CNTN2 ORF mammalian expression plasmid, N-Flag tag||RG80420-NF|
|Rat CNTN2 ORF mammalian expression plasmid, N-His tag||RG80420-NH|
|Rat CNTN2 ORF mammalian expression plasmid, N-Myc tag||RG80420-NM|
|Rat CNTN2 ORF mammalian expression plasmid, N-HA tag||RG80420-NY|
|Rat CNTN2 natural ORF mammalian expression plasmid||RG80420-UT|
|Learn more about expression Vectors|
Contactins are a subgroup of molecules belonging to the immunoglobulin superfamily that are expressed exclusively in the nervous system. The subgroup consists of six members: Contactin-1, Contactin-2(TAG-1), Contactin-3(BIG-1), BIG-2, Contactin-5(NB-2) and NB-3. Since their identification in the late 1980s, Contactin-1 and Contactin-2 have been studied extensively. Axonal expression and the neurite extension activity of Contactin-1 and Contactin-2 attracted researchers to study the function of these molecules in axon guidance during development. Contactin-1 and Contactin-2 have come to be known as the principal molecules in the function and maintenance of myelinated neurons. In contrast, the function of the other four members of this subgroup remained unknown until recently. Contactin-2, also known as CNTN2, is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. The human, rat, and chicken Contactin-2 are alternatively known as TAX1 (transiently-expressed axonal glycoprotein), TAG1 (transient axonal glycoprotein), and axonin-1, respectively. Human Contactin-2 shares approximately 91% and 75% amino acid sequence identity with rat and chicken Contactin-2, respectively. Contactin-2 is expressed by a subset of neuronal populations in the developing central nervous system (CNS) and peripheral nervous system (PNS). Contactin-2 is also expressed by oligodendrocytes and Schwann cells, which are myelinating glial cells of the CNS and PNS, respectively. Contactin-2 may play a role in the formation of axon connections in the developing nervous system. Contactin-2 is also involved in glial tumorigenesis and may provide a potential target for therapeutic intervention. During embryonic development, Contactin-2 interacts either in a homophilic, or heterophilic fashion with various transmembrane proteins.