|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat LAIR1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80415-ACG|
|Rat LAIR1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80415-ACR|
|Rat LAIR1 ORF mammalian expression plasmid, C-Flag tag||RG80415-CF|
|Rat LAIR1 ORF mammalian expression plasmid, C-His tag||RG80415-CH|
|Rat LAIR1 ORF mammalian expression plasmid, C-Myc tag||RG80415-CM|
|Rat LAIR1 ORF mammalian expression plasmid, C-HA tag||RG80415-CY|
|Rat LAIR1 Gene cDNA clone plasmid||RG80415-G|
|Rat LAIR1 ORF mammalian expression plasmid, N-Flag tag||RG80415-NF|
|Rat LAIR1 ORF mammalian expression plasmid, N-His tag||RG80415-NH|
|Rat LAIR1 ORF mammalian expression plasmid, N-Myc tag||RG80415-NM|
|Rat LAIR1 ORF mammalian expression plasmid, N-HA tag||RG80415-NY|
|Rat LAIR1 natural ORF mammalian expression plasmid||RG80415-UT|
|Learn more about expression Vectors|
Leukocyte associated Ig-like receptor-1 (LAIR1) is a surface molecule expressed on human mononuclear leukocytes that functions as an inhibitory receptor on human NK cells. In addition to NK cells, LAIR1 is expressed on T cells, B cells, macrophages, and dendritic cells. It is predicted to mediate inhibitory functions based on the presence of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in its cytoplasmic domain. Cross-linking of LAIR1 on human T cell clones results in inhibition of cytotoxicity only in T cell clones that lack CD28 and are able to spontaneously lyse certain targets in vitro. Moreover, the cytolytic activity of freshly isolated T cells, which is thought to be mainly due to "effector" T cells, can be inhibited by anti-LAIR1 mAb. Thus, LAIR1 functions as an inhibitory receptor not only on NK cells, but also on human T cells. This indicates that LAIR1 provides a mechanism of regulation of effector T cells and may play a role in the inhibition of unwanted bystander responses mediated by Ag-specific T cells.