|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat CD300A ORF mammalian expression plasmid, C-GFPSpark tag||RG80410-ACG|
|Rat CD300A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80410-ACR|
|Rat CD300A ORF mammalian expression plasmid, C-Flag tag||RG80410-CF|
|Rat CD300A ORF mammalian expression plasmid, C-His tag||RG80410-CH|
|Rat CD300A ORF mammalian expression plasmid, C-Myc tag||RG80410-CM|
|Rat CD300A ORF mammalian expression plasmid, C-HA tag||RG80410-CY|
|Rat CD300A Gene cDNA clone plasmid||RG80410-G|
|Rat CD300A ORF mammalian expression plasmid, N-Flag tag||RG80410-NF|
|Rat CD300A ORF mammalian expression plasmid, N-His tag||RG80410-NH|
|Rat CD300A ORF mammalian expression plasmid, N-Myc tag||RG80410-NM|
|Rat CD300A ORF mammalian expression plasmid, N-HA tag||RG80410-NY|
|Rat CD300A natural ORF mammalian expression plasmid||RG80410-UT|
|Learn more about expression Vectors|
Mouse CMRF35-like molecule 8, also known as CD300 antigen-like family member A, CMRF35-H9, Immunoglobulin superfamily member 12, Inhibitory receptor protein 60, NK inhibitory receptor, CD300a and CMRF35H, is a single-pass type I membrane protein which belongs to the CD300 family. The CD300 family of myeloid immunoglobulin receptors includes activating ( CD300b, CD300e ) and inhibitory members ( CD300a, CD300f ), as well as molecules presenting a negative charge within their transmembrane domain ( CD300c, CD300d ). CD300A / IGSF12 is expressed not only by natural killer (NK) cells but also by T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes and monocytes.It contains one Ig-like V-type ( immunoglobulin-like ) domain. CD300A / IGSF12 is an inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. CD300c is a functional immune receptor able to deliver activating signals upon ligation in RBL-2H3 mast cells. CD300c signaling is partially mediated by a direct association with the immune receptor tyrosine-based activation motif-bearing adaptor FcεRγ. CD300a and CD300c play an important role in the cross-regulation of TNF-alpha and IFN-alpha secretion from plasmacytoid dendritic cells (pDCs).