|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse CTSA ORF mammalian expression plasmid, C-GFPSpark tag||MG50348-ACG|
|Mouse CTSA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50348-ACR|
|Mouse CTSA ORF mammalian expression plasmid, C-Flag tag||MG50348-CF|
|Mouse CTSA ORF mammalian expression plasmid, C-His tag||MG50348-CH|
|Mouse CTSA ORF mammalian expression plasmid, C-Myc tag||MG50348-CM|
|Mouse CTSA ORF mammalian expression plasmid, C-HA tag||MG50348-CY|
|Mouse CTSA Gene cDNA clone plasmid||MG50348-M|
|Mouse CTSA ORF mammalian expression plasmid, N-Flag tag||MG50348-NF|
|Mouse CTSA ORF mammalian expression plasmid, N-His tag||MG50348-NH|
|Mouse CTSA ORF mammalian expression plasmid, N-Myc tag||MG50348-NM|
|Mouse CTSA ORF mammalian expression plasmid, N-HA tag||MG50348-NY|
|Mouse CTSA natural ORF mammalian expression plasmid||MG50348-UT|
|Learn more about expression Vectors|
Lysosomal carboxypeptidase, cathepsin A (protective protein, CathA), is a component of the lysosomal multienzyme complex along with beta-galactosidase (GAL) and sialidase Neu1, where it activates Neu1 and protects GAL and Neu1 against the rapid proteolytic degradation. Cathepsin A is a multicatalytic enzyme with deamidase and esterase in addition to carboxypeptidase activities. It was recently identified in human platelets as deamidase. In vitro, it hydrolyzes a variety of bioactive peptide hormones including tachykinins, suggesting that extralysosomal cathepsin A plays a role in regulation of bioactive peptide functions. It is a member of the alpha/beta hydrolase fold family and has been suggested to share a common ancestral relationship with other alpha/beta hydrolase fold enzymes, such as cholinesterases. Cathepsin A defects are linked to multiple forms of Galactosialidosis with a combined secondary deficiency of beta-galactosidase and neuraminidase. Cathepsin A is a key molecule in the onset of galactosialidosis and also highlight the therapeutic acts in vivo as an endothelin-1-inactivating enzyme and strongly confirm a crucial role of this enzyme in effective elastic fiber formation.