|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat ADAM17 ORF mammalian expression plasmid, C-GFPSpark tag||RG80350-ACG|
|Rat ADAM17 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80350-ACR|
|Rat ADAM17 ORF mammalian expression plasmid, C-Flag tag||RG80350-CF|
|Rat ADAM17 ORF mammalian expression plasmid, C-His tag||RG80350-CH|
|Rat ADAM17 ORF mammalian expression plasmid, C-Myc tag||RG80350-CM|
|Rat ADAM17 ORF mammalian expression plasmid, C-HA tag||RG80350-CY|
|Rat ADAM17 Gene cDNA clone plasmid||RG80350-G|
|Rat ADAM17 ORF mammalian expression plasmid, N-Flag tag||RG80350-NF|
|Rat ADAM17 ORF mammalian expression plasmid, N-His tag||RG80350-NH|
|Rat ADAM17 ORF mammalian expression plasmid, N-Myc tag||RG80350-NM|
|Rat ADAM17 ORF mammalian expression plasmid, N-HA tag||RG80350-NY|
|Rat ADAM17 natural ORF mammalian expression plasmid||RG80350-UT|
|Learn more about expression Vectors|
ADAM17 is a member of the ADAM protein family of disintegrins and metalloproteases. ADAM17 is ubiquitously expressed in the human colon, with increased activity in the colonic mucosa of patients with ulcerative colitis, a main form of inflammatory bowel disease. The expression of ADAM17 may be inhibited by ethanol. It is involved in the processing of tumor necrosis factor alpha (TNF-α) at the surface of the cell, and from within the intracellular membranes of the trans-Golgi network. ADAM17 also plays a role in the release of a diverse variety of membrane-anchored cytokines, cell adhesion molecules, receptors, ligands, and enzymes. ADAM17 may play a prominent role in the Notch signaling pathway, during the proteolytic release of the Notch intracellular domain (from the Notch1 receptor) that occurs following ligand binding.