|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat THBD ORF mammalian expression plasmid, C-GFPSpark tag||RG80348-ACG|
|Rat THBD ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80348-ACR|
|Rat THBD ORF mammalian expression plasmid, C-Flag tag||RG80348-CF|
|Rat THBD ORF mammalian expression plasmid, C-His tag||RG80348-CH|
|Rat THBD ORF mammalian expression plasmid, C-Myc tag||RG80348-CM|
|Rat THBD ORF mammalian expression plasmid, C-HA tag||RG80348-CY|
|Rat THBD Gene cDNA clone plasmid||RG80348-G|
|Rat THBD ORF mammalian expression plasmid, N-Flag tag||RG80348-NF|
|Rat THBD ORF mammalian expression plasmid, N-His tag||RG80348-NH|
|Rat THBD ORF mammalian expression plasmid, N-Myc tag||RG80348-NM|
|Rat THBD ORF mammalian expression plasmid, N-HA tag||RG80348-NY|
|Rat THBD natural ORF mammalian expression plasmid||RG80348-UT|
|Learn more about expression Vectors|
Thrombomodulin, also known as THBD(CD141), is an integral membrane protein which reduces blood coagulation by converting thrombin to an anticoagulant enzyme from a procoagulant enzyme. Thrombomodulin is expressed on the surface of endothelial cells and serves as a cofactor for thrombin. It is also expressed on human mesothelial cell, monocyte and a dendritic cell subset. Thrombomodulin functions as a cofactor in the thrombin-induced activation of protein C in the anticoagulant pathway by forming a 1:1 stoichiometric complex with thrombin. Thrombomodulin also regulates C3b inactivation by factor I. Mutations in the thrombomodulin gene have also been reported to be associated with atypical hemolytic-uremic syndrome.