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Human UBE2D1 / UBCH5 Protein (His Tag)

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Human UBE2D1/UBCH5 Protein Product Information
Synonym:E2(17)KB1, SFT, UBC4/5, UBCH5, UBCH5A
Protein Construction:A DNA sequence encoding the human UBE2D1 (P51668) (Ala 2-Met 147) was expressed, with a polyhistide tag at the N-terminus.
Species:Human
Expressed Host:E. coli
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human UBE2D1/UBCH5 Protein QC Testing
Purity:> 90 % as determined by SDS-PAGE
Endotoxin:Please contact us for more information.
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant human UBE2D1 consisting of 161 amino acids and has a calculated molecular mass of 18.3 kDa. It migrates as an approximately16 kDa band in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile 25mM Tris, 100mM NaCl, 20% glycerol, 0.05% Tween
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human UBE2D1/UBCH5 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human UBE2D1/UBCH5 Protein SDS-PAGE
Human UBE2D1 / UBCH5 Protein (His Tag) SDS-PAGE
Other UBE2D1/UBCH5 Recombinant Protein Products
11432-H07E
Human UBE2D1 References
  • Zhang L, et al. (2011) The IDOL-UBE2D complex mediates sterol-dependent degradation of the LDL receptor. Genes Dev. 25(12): 1262-74.
  • Tokumoto M, et al. (2011) Cadmium toxicity is caused by accumulation of p53 through the down-regulation of Ube2d family genes in vitro and in vivo. J Toxicol Sci. 36(2): 191-200.
  • Ohbayashi N, et al. (2008) Physical and functional interactions between ZIP kinase and UbcH5. Biochem Biophys Res Commun. 372(4): 708-12.
  • Gehrke SG, et al. (2003) UbcH5A, a member of human E2 ubiquitin-conjugating enzymes, is closely related to SFT, a stimulator of iron transport, and is up-regulated in hereditary hemochromatosis. Blood. 101(8): 3288-93.
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    Catalog: 11432-H07E-50
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"