|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat CD63 ORF mammalian expression plasmid, C-GFPSpark tag||RG80304-ACG|
|Rat CD63 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80304-ACR|
|Rat CD63 ORF mammalian expression plasmid, C-Flag tag||RG80304-CF|
|Rat CD63 ORF mammalian expression plasmid, C-His tag||RG80304-CH|
|Rat CD63 ORF mammalian expression plasmid, C-Myc tag||RG80304-CM|
|Rat CD63 ORF mammalian expression plasmid, C-HA tag||RG80304-CY|
|Rat CD63 Gene cDNA clone plasmid||RG80304-G|
|Rat CD63 ORF mammalian expression plasmid, N-Flag tag||RG80304-NF|
|Rat CD63 ORF mammalian expression plasmid, N-His tag||RG80304-NH|
|Rat CD63 ORF mammalian expression plasmid, N-Myc tag||RG80304-NM|
|Rat CD63 ORF mammalian expression plasmid, N-HA tag||RG80304-NY|
|Rat CD63 natural ORF mammalian expression plasmid||RG80304-UT|
|Learn more about expression Vectors|
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 63 (CD63) is a member of the CD family and the transmembrane 4 superfamily,also known as the tetraspanin family. CD63 is a cellular surface glycoprotein characterized by the presence of four bydrophobic domains. CD63 had functions in mediating signal transduction processes and then regulate variety of cellular processes such as cell proliferation, activation and motility. It has reported that CD63 protein associated with tumor progression and served as a blood platlet activation marker and the deficiency of this protein may be associated with Hermansky-Pudlak syndrome.