|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat CD28 ORF mammalian expression plasmid, C-GFPSpark tag||RG80302-ACG|
|Rat CD28 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80302-ACR|
|Rat CD28 ORF mammalian expression plasmid, C-Flag tag||RG80302-CF|
|Rat CD28 ORF mammalian expression plasmid, C-His tag||RG80302-CH|
|Rat CD28 ORF mammalian expression plasmid, C-Myc tag||RG80302-CM|
|Rat CD28 ORF mammalian expression plasmid, C-HA tag||RG80302-CY|
|Rat CD28 Gene cDNA clone plasmid||RG80302-G|
|Rat CD28 ORF mammalian expression plasmid, N-Flag tag||RG80302-NF|
|Rat CD28 ORF mammalian expression plasmid, N-His tag||RG80302-NH|
|Rat CD28 ORF mammalian expression plasmid, N-Myc tag||RG80302-NM|
|Rat CD28 ORF mammalian expression plasmid, N-HA tag||RG80302-NY|
|Rat CD28 natural ORF mammalian expression plasmid||RG80302-UT|
|Learn more about expression Vectors|
CD28 (Cluster of Differentiation 28) is a disulphide-bonded glycoprotein belonging to the immunoglobulin (Ig) superfamily, and structurally consists of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. Mouse CD28 is constitutively expressed on the surface of all murine T cells and on developing thymocytes as disulfide-linked homodimers or as monomers. CD28 can binds the B7-1 and B7-2 ligand, and together perform important functions in the T and B cell response pathways. B7/CD28 family members, which can augment or antagonize T-cell receptor signaling, in the regulation of central and peripheral T-cell tolerance. CD28 is thus involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.