|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse CD25 / IL2Ra ORF mammalian expression plasmid, C-GFPSpark tag||MG50292-ACG|
|Mouse CD25 / IL2Ra ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50292-ACR|
|Mouse CD25 / IL2Ra ORF mammalian expression plasmid, C-Flag tag||MG50292-CF|
|Mouse CD25 / IL2Ra ORF mammalian expression plasmid, C-His tag||MG50292-CH|
|Mouse CD25 / IL2Ra ORF mammalian expression plasmid, C-Myc tag||MG50292-CM|
|Mouse CD25 / IL2Ra ORF mammalian expression plasmid, C-HA tag||MG50292-CY|
|Mouse CD25 / IL2Ra Gene cDNA clone plasmid||MG50292-M|
|Mouse CD25 / IL2Ra ORF mammalian expression plasmid, N-Flag tag||MG50292-NF|
|Mouse CD25 / IL2Ra ORF mammalian expression plasmid, N-His tag||MG50292-NH|
|Mouse CD25 / IL2Ra ORF mammalian expression plasmid, N-Myc tag||MG50292-NM|
|Mouse CD25 / IL2Ra ORF mammalian expression plasmid, N-HA tag||MG50292-NY|
|Mouse CD25 / IL2Ra natural ORF mammalian expression plasmid||MG50292-UT|
|Learn more about expression Vectors|
CD25 (alpha-chain of IL-2 receptor, or IL2RA), is a type I transmembrane glycoprotein with a signal peptide, an extracellular region, a transmembrane region, and a cytoplasmic domain. IL2RA is expressed on activated T cells and regulatory T cells, and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covelently with the IL2 receptor beta and gamma chain, and subsequently initiates the intacellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling. The IL2RA (CD25) gene is a substantial component of the high-affinity receptor molecule highly expressed by activated T lymphocytes. Recently, a strong evidence was obtained for the involvement of IL-2RA in conferring susceptibility to type 1 diabetes (T1D). Cancer growth and development is associated with the stimulation of the innate immune system, including enhanced interleukin 2 receptor (IL-2R) expression in immune cells and its shedding into the circulation in a soluble form of sIL-2Ralpha. In most haematological malignancies, including different types of leukaemias and lymphomas, sIL-2Ralpha has been found to be released directly from the surface of neoplastic cells thus reflecting the tumour bulk, turnover and activity. Several studies have proved that not only lymphoid cancer cells, but also some non-lymphoid cancer cells, express IL-2R on their surface. They include malignant melanoma and carcinomas of the kidney, head and neck, oesophagus and lung. Thus, sIL-2Ralpha is elevated in most proliferative disturbances of the hematopoietic system and in many solid tumors.