|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat CDH2 ORF mammalian expression plasmid, C-GFPSpark tag||RG80275-ACG|
|Rat CDH2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80275-ACR|
|Rat CDH2 ORF mammalian expression plasmid, C-Flag tag||RG80275-CF|
|Rat CDH2 ORF mammalian expression plasmid, C-His tag||RG80275-CH|
|Rat CDH2 ORF mammalian expression plasmid, C-Myc tag||RG80275-CM|
|Rat CDH2 ORF mammalian expression plasmid, C-HA tag||RG80275-CY|
|Rat CDH2 Gene cDNA clone plasmid||RG80275-G|
|Rat CDH2 ORF mammalian expression plasmid, N-Flag tag||RG80275-NF|
|Rat CDH2 ORF mammalian expression plasmid, N-His tag||RG80275-NH|
|Rat CDH2 ORF mammalian expression plasmid, N-Myc tag||RG80275-NM|
|Rat CDH2 ORF mammalian expression plasmid, N-HA tag||RG80275-NY|
|Rat CDH2 natural ORF mammalian expression plasmid||RG80275-UT|
|Learn more about expression Vectors|
Cadherins are calcium dependent cell adhesion proteins, and they preferentially interact with themselves in a homophilic manner in connecting cells. Cadherin 2 (CDH2), also known as N-Cadherin (neuronal) (NCAD), is a single-pass tranmembrane protein and a cadherin containing 5 cadherin domains. N-Cadherin displays a ubiquitous expression pattern but with different expression levels between endocrine cell types. CDH2 (NCAD) has been shown to play an essential role in normal neuronal development, which is implicated in an array of processes including neuronal differentiation and migration, and axon growth and fasciculation. In addition, N-Cadherin expression was upregulated in human HSC during activation in culture, and function or expression blocking of N-Cadherin promoted apoptosis. During apoptosis, N-Cadherin was cleaved into 20-100 kDa fragments. It may provide a novel target for therapies that are directed toward intimal proliferative disorders, including restenosis and vascular bypass graft failure. N-Cadherin is associated with tumor aggressiveness and metastatic potential and may contribute to tumor progression.