|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat CD27 ORF mammalian expression plasmid, C-GFPSpark tag||RG80223-ACG|
|Rat CD27 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80223-ACR|
|Rat CD27 ORF mammalian expression plasmid, C-Flag tag||RG80223-CF|
|Rat CD27 ORF mammalian expression plasmid, C-His tag||RG80223-CH|
|Rat CD27 ORF mammalian expression plasmid, C-Myc tag||RG80223-CM|
|Rat CD27 ORF mammalian expression plasmid, C-HA tag||RG80223-CY|
|Rat CD27 Gene cDNA clone plasmid||RG80223-G|
|Rat CD27 ORF mammalian expression plasmid, N-Flag tag||RG80223-NF|
|Rat CD27 ORF mammalian expression plasmid, N-His tag||RG80223-NH|
|Rat CD27 ORF mammalian expression plasmid, N-Myc tag||RG80223-NM|
|Rat CD27 ORF mammalian expression plasmid, N-HA tag||RG80223-NY|
|Rat CD27 natural ORF mammalian expression plasmid||RG80223-UT|
|Learn more about expression Vectors|
CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has important role in generation of T cell immunity, and is an apparently robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.