|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat ALCAM ORF mammalian expression plasmid, C-GFPSpark tag||RG80221-ACG|
|Rat ALCAM ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80221-ACR|
|Rat ALCAM ORF mammalian expression plasmid, C-Flag tag||RG80221-CF|
|Rat ALCAM ORF mammalian expression plasmid, C-His tag||RG80221-CH|
|Rat ALCAM ORF mammalian expression plasmid, C-Myc tag||RG80221-CM|
|Rat ALCAM ORF mammalian expression plasmid, C-HA tag||RG80221-CY|
|Rat ALCAM Gene cDNA clone plasmid||RG80221-G|
|Rat ALCAM ORF mammalian expression plasmid, N-Flag tag||RG80221-NF|
|Rat ALCAM ORF mammalian expression plasmid, N-His tag||RG80221-NH|
|Rat ALCAM ORF mammalian expression plasmid, N-Myc tag||RG80221-NM|
|Rat ALCAM ORF mammalian expression plasmid, N-HA tag||RG80221-NY|
|Rat ALCAM natural ORF mammalian expression plasmid||RG80221-UT|
|Learn more about expression Vectors|
Activated leukocyte cell adhesion molecule (ALCAM)/Cluster of differentiation (CD166) is a type I transmembrane cell adhesion molecule belonging to the Ig superfamily and a ligand for CD6 that is expressed on T lymphocytes. The extracellular domain of ALCAM contains five Ig-like domains (three Ig-like C2-type domains and two Ig-like V-type domains), of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation. ALCAM mediates both heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. ALCAM/CD6 interaction plays a role in T cell development and T cell regulation, as well as in the binding of T- and B-cells to activated leukocytes. Recently, homophilic (ALCAM-ALCAM) adhesion was shown to play important roles in tight cell-to-cell interaction and regulation of stem cell differentiation. While expressed in a wide variety of tissues, ALCAM is usually restricted to subsets of cells involved in dynamic growth and/or migration, including neural development, branching organ development, hematopoiesis, immune response and tumor progression. And CD166 is regarded as a potential novel breast cancer indicator and therapeutic target.