|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat IL4R ORF mammalian expression plasmid, C-GFPSpark tag||RG80198-ACG|
|Rat IL4R ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80198-ACR|
|Rat IL4R ORF mammalian expression plasmid, C-Flag tag||RG80198-CF|
|Rat IL4R ORF mammalian expression plasmid, C-His tag||RG80198-CH|
|Rat IL4R ORF mammalian expression plasmid, C-Myc tag||RG80198-CM|
|Rat IL4R ORF mammalian expression plasmid, C-HA tag||RG80198-CY|
|Rat IL4R Gene cDNA clone plasmid||RG80198-G|
|Rat IL4R ORF mammalian expression plasmid, N-Flag tag||RG80198-NF|
|Rat IL4R ORF mammalian expression plasmid, N-His tag||RG80198-NH|
|Rat IL4R ORF mammalian expression plasmid, N-Myc tag||RG80198-NM|
|Rat IL4R ORF mammalian expression plasmid, N-HA tag||RG80198-NY|
|Rat IL4R natural ORF mammalian expression plasmid||RG80198-UT|
|Learn more about expression Vectors|
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD124, also known as interleukin 4 receptor (IL4R), is a typeⅠ transmembrane protein that can regulate IgE antibody production in B cells through binding to interleukin 4 and interleukin 13 and promote differentiation of Th2 cells through binding to interleukin 4. The membrane-bound form of CD124 can be hydrolyzed to soluble form which can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells.