|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat IL2RG ORF mammalian expression plasmid, C-GFPSpark tag||RG80197-ACG|
|Rat IL2RG ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80197-ACR|
|Rat IL2RG ORF mammalian expression plasmid, C-Flag tag||RG80197-CF|
|Rat IL2RG ORF mammalian expression plasmid, C-His tag||RG80197-CH|
|Rat IL2RG ORF mammalian expression plasmid, C-Myc tag||RG80197-CM|
|Rat IL2RG ORF mammalian expression plasmid, C-HA tag||RG80197-CY|
|Rat IL2RG Gene cDNA clone plasmid||RG80197-G|
|Rat IL2RG ORF mammalian expression plasmid, Flag tag||RG80197-G-F|
|Rat IL2RG ORF mammalian expression plasmid, N-Flag tag||RG80197-NF|
|Rat IL2RG ORF mammalian expression plasmid, N-His tag||RG80197-NH|
|Rat IL2RG ORF mammalian expression plasmid, N-Myc tag||RG80197-NM|
|Rat IL2RG ORF mammalian expression plasmid, N-HA tag||RG80197-NY|
|Rat IL2RG natural ORF mammalian expression plasmid||RG80197-UT|
|Learn more about expression Vectors|
The common gamma chain (γc) (or CD132), also known as interleukin-2 receptor subunit gamma or IL2RG, is a member of the type I cytokine receptor family expressed on most lymphocyte (white blood cell) populations, and its gene is found on the X-chromosome of mammals. The common gamma chain (γc) (or IL2RG), is a cytokine receptor sub-unit that is common to the receptor complexes for at least six different interleukin receptors: IL-2, IL-4, IL-7, IL-9, IL-15 and interleukin-21 receptor. It is a component of multiple cytokine receptors that are essential for lymphocyte development and function. X-linked severe combined immunodeficiency (XSCID) is a rare and potentially fatal disease caused by mutations of IL2RG, the gene encoding IL2RG. IL2RG was demonstrated to be a component of the IL-4 receptor on the basis of chemical cross-linking data, the ability of IL2RG to augment IL-4 binding affinity. The observation that IL-2R gamma is a functional component of the IL-4 receptor, together with the finding that IL-2R gamma associates with the IL-7 receptor, begins to elucidate why deficiency of this common gamma chain (gamma c) has a profound effect on lymphoid function and development, as seen in X-linked severe combined immunodeficiency.