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Mouse EGFR Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-tagged

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EGFRcDNA Clone Product Information
Gene_bank_ref_id:NM_207655.2
cDNA Size:3633
cDNA Description:ORF Clone of Mus musculus epidermal growth factor receptor DNA.
Gene Synonym:Wa5, wa2, Erbb, Errp, wa-2, AI552599, 9030024J15Rik, Egfr
Species:Mouse
Vector:pCMV3-N-His
Restriction Site:
Tag Sequence:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequence Description:
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pCMV3-SP-N-His (suitable for secretary and membane protein expession) Vector Information
 
Vector Name pCMV3-SP-N-His
Vector Size 6149bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive, Stable / Transient
Promoter CMV
Antibiotic Resistance Kanamycin
Selection In Mammalian Cells Hygromycin
Protein Tag His
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)

pCMV3-SP-N-His (suitable for secretary and membane protein expession) Physical Map
Schematic of pCMV3-SP-N-His (suitable for secretary and membane protein expession) Multiple Cloning Sites

His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

Mouse EGFR Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-tagged  on other vectors
Mouse EGFR Gene cDNA Clone (full-length ORF Clone), expression ready, C-GFPSpark-taggedMG51091-ACG$475
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Mouse EGFR Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-taggedMG51091-CF$445
Mouse EGFR Gene cDNA Clone (full-length ORF Clone), expression ready, C-His-tagged MG51091-CH$445
Mouse EGFR Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-tagged MG51091-CM$445
Mouse EGFR Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-tagged MG51091-CY$445
Mouse EGFR Gene cDNA Clone (full-length ORF Clone) MG51091-G$395
Mouse EGFR Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-taggedMG51091-G-F$645
Mouse EGFR Gene cDNA Clone (full-length ORF Clone), expression ready, His-taggedMG51091-G-H$645
Mouse EGFR Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedMG51091-G-N$645
Mouse EGFR Gene cDNA Clone (full-length ORF Clone), expression ready, HA-taggedMG51091-G-Y$645
Mouse EGFR Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-taggedMG51091-NF$445
Mouse EGFR Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-tagged MG51091-NH$445
Mouse EGFR Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-tagged MG51091-NM$445
Mouse EGFR Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-tagged MG51091-NY$445
Mouse EGFR Gene cDNA Clone (full-length ORF Clone), expression ready, untagged MG51091-UT$445
 Learn more about expression Vectors
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Background

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.

References
  • Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
  • Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
  • Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.
  • Size / Price
    Catalog:MG51091-NH
    List Price: $445.00  (Save $0.00)
    Price:$445.00      [How to order]
    Availability2-3 weeks
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