|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse NT5E ORF mammalian expression plasmid, C-GFPSpark tag||MG50231-ACG|
|Mouse NT5E ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50231-ACR|
|Mouse NT5E ORF mammalian expression plasmid, C-Flag tag||MG50231-CF|
|Mouse NT5E ORF mammalian expression plasmid, C-His tag||MG50231-CH|
|Mouse NT5E ORF mammalian expression plasmid, C-Myc tag||MG50231-CM|
|Mouse NT5E ORF mammalian expression plasmid, C-HA tag||MG50231-CY|
|Mouse NT5E Gene cDNA clone plasmid||MG50231-M|
|Mouse NT5E natural ORF mammalian expression plasmid||MG50231-M-N|
|Mouse NT5E ORF mammalian expression plasmid, N-Flag tag||MG50231-NF|
|Mouse NT5E ORF mammalian expression plasmid, N-His tag||MG50231-NH|
|Mouse NT5E ORF mammalian expression plasmid, N-Myc tag||MG50231-NM|
|Mouse NT5E ORF mammalian expression plasmid, N-HA tag||MG50231-NY|
|Mouse NT5E natural ORF mammalian expression plasmid||MG50231-UT|
|Learn more about expression Vectors|
5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein which belongs to the 5'-nucleotidase family. CD73 is a glycosyl phosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signalling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1 and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purigenic receptors to induce cellular responses. CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.