|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat TNFRSF11B ORF mammalian expression plasmid, C-GFPSpark tag||RG80159-ACG|
|Rat TNFRSF11B ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80159-ACR|
|Rat TNFRSF11B ORF mammalian expression plasmid, C-Flag tag||RG80159-CF|
|Rat TNFRSF11B ORF mammalian expression plasmid, C-His tag||RG80159-CH|
|Rat TNFRSF11B ORF mammalian expression plasmid, C-Myc tag||RG80159-CM|
|Rat TNFRSF11B ORF mammalian expression plasmid, C-HA tag||RG80159-CY|
|Rat TNFRSF11B Gene cDNA clone plasmid||RG80159-G|
|Rat TNFRSF11B ORF mammalian expression plasmid, N-Flag tag||RG80159-NF|
|Rat TNFRSF11B ORF mammalian expression plasmid, N-His tag||RG80159-NH|
|Rat TNFRSF11B ORF mammalian expression plasmid, N-Myc tag||RG80159-NM|
|Rat TNFRSF11B ORF mammalian expression plasmid, N-HA tag||RG80159-NY|
|Rat TNFRSF11B natural ORF mammalian expression plasmid||RG80159-UT|
|Learn more about expression Vectors|
Osteoprotegerin or TNFRSF11B is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. Osteoprotegerin/TNFRSF11B acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. This protein may inhibit the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. Osteoprotegerin/TNFRSF11B also play a role in preventing arterial calcification, act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis.