|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|Mouse INSR ORF mammalian expression plasmid, C-GFPSpark tag||MG51062-ACG|
|Mouse INSR ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51062-ACR|
|Mouse INSR ORF mammalian expression plasmid, C-Flag tag||MG51062-CF|
|Mouse INSR ORF mammalian expression plasmid, C-His tag||MG51062-CH|
|Mouse INSR ORF mammalian expression plasmid, C-Myc tag||MG51062-CM|
|Mouse INSR ORF mammalian expression plasmid, C-HA tag||MG51062-CY|
|Mouse INSR Gene cDNA clone plasmid||MG51062-G|
|Mouse INSR ORF mammalian expression plasmid, N-Flag tag||MG51062-NF|
|Mouse INSR ORF mammalian expression plasmid, N-His tag||MG51062-NH|
|Mouse INSR ORF mammalian expression plasmid, N-Myc tag||MG51062-NM|
|Mouse INSR ORF mammalian expression plasmid, N-HA tag||MG51062-NY|
|Mouse INSR natural ORF mammalian expression plasmid||MG51062-UT|
|Learn more about expression Vectors|
INSR (Insulin receptor), also known as CD220, is a transmembrane receptor that is activated by insulin. INSR belongs to theprotein kinase superfamily, and exists as a tetramer consisting of two alpha subunits and two beta subunits linked by disulfide bonds. The alpha and beta subunits are encoded by a single INSR gene, and the beta subunits pass through the cellular membrane. As the receptor for insulin with tyrosine-protein kinase activity, INSR associates with downstream mediators upon binding to insulin, including IRS1 (insulin receptor substrate 1) and phosphatidylinositol 3'-kinase (PI3K). IRS-1 binding and phosphorylation eventually leads to an increase in the high affinity glucose transporter (Glut4) molecules on the outer membrane of insulin-responsive tissues. INSR isoform long and isoform short are expressed in the peripheral nerve, kidney, liver, striated muscle, fibroblasts and skin, and is found as a hybrid receptor with IGF1R which also binds IGF1 in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibrobasts, spleen and placenta. Defects in Insulin Receptor/INSR are the cause of Rabson-Mendenhall syndrome (Mendenhall syndrome), insulin resistance (Ins resistance), leprechaunism (Donohue syndrome), and familial hyperinsulinemic hypoglycemia 5 (HHF5). It may also be associated with noninsulin-dependent diabetes mellitus (NIDDM).