|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat BMP7 ORF mammalian expression plasmid, C-GFPSpark tag||RG80120-ACG|
|Rat BMP7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80120-ACR|
|Rat BMP7 ORF mammalian expression plasmid, C-Flag tag||RG80120-CF|
|Rat BMP7 ORF mammalian expression plasmid, C-His tag||RG80120-CH|
|Rat BMP7 ORF mammalian expression plasmid, C-Myc tag||RG80120-CM|
|Rat BMP7 ORF mammalian expression plasmid, C-HA tag||RG80120-CY|
|Rat BMP7 Gene cDNA clone plasmid||RG80120-G|
|Rat BMP7 ORF mammalian expression plasmid, N-Flag tag||RG80120-NF|
|Rat BMP7 ORF mammalian expression plasmid, N-His tag||RG80120-NH|
|Rat BMP7 ORF mammalian expression plasmid, N-Myc tag||RG80120-NM|
|Rat BMP7 ORF mammalian expression plasmid, N-HA tag||RG80120-NY|
|Rat BMP7 natural ORF mammalian expression plasmid||RG80120-UT|
|Learn more about expression Vectors|
BMP-7 (Bone morphogenetic protein 7), also known as osteogenic protein 1 (OP-1), is a bone morphogenetic protein which belongs to the TGF-β superfamily. OP-1 is expressed in the brain, kidneys and bladder. BMP-7 may be involved in bone homeostasis. Osteogenic protein 1 plays a key role in the transformation of mesenchymal cells into bone and cartilage. The phosphorylation of SMAD1 and SMAD5 can be induced by BMP-7, which in turn induce transcription of numerous osteogenic genes. BMP-7 treatment can also induce all of the genetic markers of osteoblast differentiation in many cell types. The expression of BMP-7 causes ventral phenotypes while its complete inhibition creates a dorsal phenotype. Human recombinant BMP-7 protein can be used to aid in the fusion of vertebral bodies to prevent neurologic trauma. It also functions in the treatment of tibial non-union, frequently in cases where a bone graft has failed. It is found that BMP7 has the potential for treatment of chronic kidney disease.