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Human MGAT5 / GGNT5 Protein (His Tag)

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Human MGAT5 Protein Product Information
Synonym:GNT-V, GNT-VA
Protein Construction:A DNA sequence encoding the human MGAT5 (NP_002401.1) C-terminal segment (Leu 189-Leu 741) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the terminus.
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human MGAT5 Protein QC Testing
Purity:> 98 % as determined by SDS-PAGE
Bio-Activity:Measured by its ability to transfer NAcetylαDglucosamine from UDPNAcetylαDglucosamine to a biantennary Nlinked core pentasaccharide in a CD39L3 coupled assay. The specific activity is >10pmoles/min/μg
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Leu 189
Molecule Mass:The recombinant human MGAT5 consists of 564 amino acids and predictes a molecular mass of 65 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh MGAT5 is approximately 60-65 kDa.
Formulation:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human MGAT5 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Other MGAT5 Recombinant Protein Products
MGAT5 Background

Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A, also known as Alpha-mannoside beta-1,6-N-acetylglucosaminyl-transferase, Mannoside acetylglucosaminyltransferase 5, N-acetylglucosaminyl-transferase V, MGAT5 and GGNT5, is a single-pass type I I membrane protein which belongs to the glycosyltransferase 18 family. MGAT5 / GGNT5 catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. The central nervous system (CNS) is rich in glycoconjugates, located on cell surface and in extracellular matrix. MGAT5 / GGNT5 modification of complex-type N-glycans on CNS glycoproteins is involved in the regulation of depression-like behavior. Inhibitors of MGAT5 / GGNT5 might be useful in the treatment of malignancies by targeting their dependency on focal adhesion signaling for growth and metastasis.

Human MGAT5 References
  • Morgan,R. et al., 2004,J Immunol  173 (12): 7200-8.
  • Cheung,P. et al., 2007,Glycobiology  17 (7): 767-73.
  • Li,D. et al., 2008, J Immunol 180 (5): 3158-65.
  • Soleimani,L. et al., 2008, Genes Brain Behav. 7 (3):334-43.
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    Catalog: 11373-H08H-20
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