|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat TGFB2 ORF mammalian expression plasmid, C-GFPSpark tag||RG80119-ACG|
|Rat TGFB2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80119-ACR|
|Rat TGFB2 ORF mammalian expression plasmid, C-Flag tag||RG80119-CF|
|Rat TGFB2 ORF mammalian expression plasmid, C-His tag||RG80119-CH|
|Rat TGFB2 ORF mammalian expression plasmid, C-Myc tag||RG80119-CM|
|Rat TGFB2 ORF mammalian expression plasmid, C-HA tag||RG80119-CY|
|Rat TGFB2 Gene cDNA clone plasmid||RG80119-G|
|Rat TGFB2 ORF mammalian expression plasmid, N-Flag tag||RG80119-NF|
|Rat TGFB2 ORF mammalian expression plasmid, N-His tag||RG80119-NH|
|Rat TGFB2 ORF mammalian expression plasmid, N-Myc tag||RG80119-NM|
|Rat TGFB2 ORF mammalian expression plasmid, N-HA tag||RG80119-NY|
|Rat TGFB2 natural ORF mammalian expression plasmid||RG80119-UT|
|Learn more about expression Vectors|
TGF beta 2 (Transforming growth factor beta 2), an extracellular glycosylated protein, which belongs to the TGF-beta family. TGF-beta regulates key mechanisms of tumor development, namely immunosuppression, metastasis, angiogenesis, and proliferation. TGF beta 2 suppression is a promising therapeutic approach for malignant tumor therapy. The signaling pathway of TGF beta 2/Smad plays an important role in the pathological process in posterior capsule opacification (PCO) after cataract surgery. Silencing Smad2 and Smad3 efficiently blocked the effect of TGF beta 2 on cell proliferation, migration, and extracellular matrix production. TGF beta 2 activation of MEKK3/ERK1/2/5 signaling modulates Has2 expression and hyaluronan (HA) production leading to the induction of epithelial to mesenchymal transformation (EMT) events. In addition, the upregulation of the TGF beta 2 level is a common pathological feature of Alzheimer's disease (AD) brains and suggests that it may be closely linked to the development of neuronal death related to AD.