|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat TGFB1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80116-ACG|
|Rat TGFB1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80116-ACR|
|Rat TGFB1 ORF mammalian expression plasmid, C-Flag tag||RG80116-CF|
|Rat TGFB1 ORF mammalian expression plasmid, C-His tag||RG80116-CH|
|Rat TGFB1 ORF mammalian expression plasmid, C-Myc tag||RG80116-CM|
|Rat TGFB1 ORF mammalian expression plasmid, C-HA tag||RG80116-CY|
|Rat TGFB1 Gene cDNA clone plasmid||RG80116-G|
|Rat TGFB1 ORF mammalian expression plasmid, N-Flag tag||RG80116-NF|
|Rat TGFB1 ORF mammalian expression plasmid, N-His tag||RG80116-NH|
|Rat TGFB1 ORF mammalian expression plasmid, N-Myc tag||RG80116-NM|
|Rat TGFB1 ORF mammalian expression plasmid, N-HA tag||RG80116-NY|
|Rat TGFB1 natural ORF mammalian expression plasmid||RG80116-UT|
|Learn more about expression Vectors|
TGF-beta 1 is a member of the transforming growth factor beta (TGF-beta) family. The transforming growth factor-beta family of polypeptides are involved in the regulation of cellular processes, including cell division, differentiation, motility, adhesion and death. TGF-beta 1 positively and negatively regulates many other growth factors. It inhibits the secretion and activity of many other cytokines including interferon-γ, tumor necrosis factor-alpha and various interleukins. It can also decrease the expression levels of cytokine receptors. Meanwhile, TGF-beta 1 also increases the expression of certain cytokines in T cells and promotes their proliferation, particularly if the cells are immature. TGF-beta 1 also inhibits proliferation and stimulates apoptosis of B cells, and plays a role in controlling the expression of antibody, transferrin and MHC class II proteins on immature and mature B cells. As for myeloid cells, TGF-beta 1can inhibit their proliferation and prevent their production of reactive oxygen and nitrogen intermediates. However, as with other cell types, TGF-beta 1 also has the opposite effect on cells of myeloid origin. TGF-beta 1 is a multifunctional protein that controls proliferation, differentiation and other functions in many cell types. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Once cells lose their sensitivity to TGF-beta1-mediated growth inhibition, autocrine TGF-beta signaling can promote tumorigenesis. Elevated levels of TGF-beta1 are often observed in advanced carcinomas, and have been correlated with increased tumor invasiveness and disease progression.