|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse KIRREL1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50193-ACG|
|Mouse KIRREL1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50193-ACR|
|Mouse KIRREL1 ORF mammalian expression plasmid, C-Flag tag||MG50193-CF|
|Mouse KIRREL1 ORF mammalian expression plasmid, C-His tag||MG50193-CH|
|Mouse KIRREL1 ORF mammalian expression plasmid, C-Myc tag||MG50193-CM|
|Mouse KIRREL1 ORF mammalian expression plasmid, C-HA tag||MG50193-CY|
|Mouse KIRREL1 Gene cDNA clone plasmid||MG50193-M|
|Mouse KIRREL1 ORF mammalian expression plasmid, N-Flag tag||MG50193-NF|
|Mouse KIRREL1 ORF mammalian expression plasmid, N-His tag||MG50193-NH|
|Mouse KIRREL1 ORF mammalian expression plasmid, N-Myc tag||MG50193-NM|
|Mouse KIRREL1 ORF mammalian expression plasmid, N-HA tag||MG50193-NY|
|Mouse KIRREL1 natural ORF mammalian expression plasmid||MG50193-UT|
|Learn more about expression Vectors|
NEPH1 (KIRREL1) belongs to a family of three closely related transmembrane proteins of the Ig superfamily with a structure similar to that of nephrin. All three Neph proteins share a conserved podocin-binding motif; mutation of a centrally located tyrosine residue dramatically lowers the affinity of Neph1 for podocin. Neph1 triggers AP-1 activation similarly to nephrin but requires the presence of Tec family kinases for efficient transactivation. Neph1 consists of a signal peptide, five Ig-like C2-type domains with the middle domain overlapping with a PKD-like domain, an RGD sequence, a transmembrane domain and a cytoplasmic tail, which is expressed in slit diaphragm domains of podocytes and in vertebrate and invertebrate nervous systems. Neph1 is abundantly expressed in the kidney, specifically expressed in podocytes of kidney glomeruli, and plays a significant role in the normal development and function of the glomerular permeability. Neph1 interacts with nephrin in vitro and in vivo, and able to stimulate transcriptional activation in a model system, such as the activation the transcription factor AP-1 via the stimulation of a MAPK module. Neph1 is crucial for the integrity of the slit diaphragm, as Neph1 gene knockout mice results in effacement of glomerular podocytes, heavy proteinuria, and early postnatal death.