|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat LCN2 / NGAL ORF mammalian expression plasmid, C-GFPSpark tag||RG80058-ACG|
|Rat LCN2 / NGAL ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80058-ACR|
|Rat LCN2 / NGAL ORF mammalian expression plasmid, C-Flag tag||RG80058-CF|
|Rat LCN2 / NGAL ORF mammalian expression plasmid, C-His tag||RG80058-CH|
|Rat LCN2 / NGAL ORF mammalian expression plasmid, C-Myc tag||RG80058-CM|
|Rat LCN2 / NGAL ORF mammalian expression plasmid, C-HA tag||RG80058-CY|
|Rat LCN2 / NGAL Gene cDNA clone plasmid||RG80058-M|
|Rat LCN2 / NGAL ORF mammalian expression plasmid, N-Flag tag||RG80058-NF|
|Rat LCN2 / NGAL ORF mammalian expression plasmid, N-His tag||RG80058-NH|
|Rat LCN2 / NGAL ORF mammalian expression plasmid, N-Myc tag||RG80058-NM|
|Rat LCN2 / NGAL ORF mammalian expression plasmid, N-HA tag||RG80058-NY|
|Rat LCN2 / NGAL natural ORF mammalian expression plasmid||RG80058-UT|
|Learn more about expression Vectors|
Lipocalin-2 (LCN2), also known as neutrophil gelatinase-associated lipocalin (NGAL), is a 25 kDa protein belonging to the lipocalin superfamily. It was initially found in activated neutrophils, however, many other cells, like kidney tubular cells, may produce NGAL in response to various insults. This protein is released from injured tubular cells after various damaging stimuli, is already known by nephrologists as one of the most promising biomarkers of incoming Acute Kidney Injury (AKI). Recent evidence also suggests its role as a biomarker in a variety of other renal and non-renal conditions. Moreover, recent studies seem to suggest a potential involvement of this factor also in the genesis and progression of chronic kidney diseases. NGAL is the first known mammalian protein which specifically binds organic molecules called siderophores, which are high-affinity iron chelators. NGAL, first known as an antibacterial factor of natural immunity, and an acute phase protein, is currently one of the most interesting and enigmatic proteins involved in the process of tumor development. acting as an intracellular iron carrier and protecting MMP9 from proteolytic degradation, NGAL has a clear pro-tumoral effect, as has already been observed in different tumors (e.g. breast, stomach, oesophagus, brain) in humans. In thyroid carcinomas, NGAL is strongly induced by NF-kB, an important factor involved both in tumor growth and in the link between chronic inflammation and neoplastic development. Thus, Lipocalin-2 (LCN2/NGAL) has been implicated in a variety of processes including cell differentiation, proliferation, survival and morphogenesis.