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pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony screening (i.e., a-complementation), as well as a pUC origin of replication.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Rat SPP1 ORF mammalian expression plasmid, C-GFPSpark tag||RG81203-ACG|
|Rat SPP1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81203-ACR|
|Rat SPP1 ORF mammalian expression plasmid, C-Flag tag||RG81203-CF|
|Rat SPP1 ORF mammalian expression plasmid, C-His tag||RG81203-CH|
|Rat SPP1 ORF mammalian expression plasmid, C-Myc tag||RG81203-CM|
|Rat SPP1 ORF mammalian expression plasmid, C-HA tag||RG81203-CY|
|Rat SPP1 ORF mammalian expression plasmid, N-Flag tag||RG81203-NF|
|Rat SPP1 ORF mammalian expression plasmid, N-His tag||RG81203-NH|
|Rat SPP1 ORF mammalian expression plasmid, N-Myc tag||RG81203-NM|
|Rat SPP1 ORF mammalian expression plasmid, N-HA tag||RG81203-NY|
|Rat SPP1 Gene cDNA clone plasmid||RG81203-U|
|Rat SPP1 natural ORF mammalian expression plasmid||RG81203-UT|
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Osteopontin, also known as Secreted phosphoprotein 1, Bone sialoprotein 1, BSP-1, OPN, and SPP1, is a member of the osteopontin family and a SIBLING glycoprotein. Osteopontin has been classified as T-helper 1 cytokine and thus believed to exacerbate inflammation in several chronic inflammatory diseases, including atherosclerosis. Besides proinflammatory functions, physiologically Osteopontin is a potent inhibitor of mineralization, it prevents ectopic calcium deposits and is a potent inducible inhibitor of vascular calcification. Osteopontin is expressed and secreted by various cells, and has a role in cell adhesion, chemotaxis, prevention of apoptosis, invasion, migration and anchorage-independent growth of tumor cells. Osteopontin recruitment functions of inflammatory cells are thought to be mediated through its adhesive domains, especially the arginine-glycine-aspartate (RGD) sequence that interacts with several integrin heterodimers. Osteopontin has emerged as a potential biomarker and mediator in cardiovascular disease. In the context of atherosclerosis, OPN is generally regarded as a proinflammatory and proatherogenic molecule. However, the role of OPN in vascular calcification (VC), which is closely related to chronic and active inflammation, is that of a negative regulator because it is an inhibitor of calcification and an active inducer of decalcification. Extensive research has demonstrated the pivotal participation of Osteopontin in the regulation of cell signaling which controls neoplastic and malignant transformation. The elevated expression of Osteopontin has been observed in a variety of cancers. It has been linked with tumor metastasis and signifies a poor prognosis for the patient.