|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat CNTF ORF mammalian expression plasmid, C-GFPSpark tag||RG80012-ACG|
|Rat CNTF ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80012-ACR|
|Rat CNTF ORF mammalian expression plasmid, N-GFPSpark tag||RG80012-ANG|
|Rat CNTF ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80012-ANR|
|Rat CNTF ORF mammalian expression plasmid, C-Flag tag||RG80012-CF|
|Rat CNTF ORF mammalian expression plasmid, C-His tag||RG80012-CH|
|Rat CNTF ORF mammalian expression plasmid, C-Myc tag||RG80012-CM|
|Rat CNTF ORF mammalian expression plasmid, C-HA tag||RG80012-CY|
|Rat CNTF Gene cDNA clone plasmid||RG80012-M|
|Rat CNTF ORF mammalian expression plasmid, N-Flag tag||RG80012-NF|
|Rat CNTF ORF mammalian expression plasmid, N-His tag||RG80012-NH|
|Rat CNTF ORF mammalian expression plasmid, N-Myc tag||RG80012-NM|
|Rat CNTF ORF mammalian expression plasmid, N-HA tag||RG80012-NY|
|Rat CNTF natural ORF mammalian expression plasmid||RG80012-UT|
|Learn more about expression Vectors|
Ciliary neurotrophic factor (CNTF) is a member of the cytokine family. It is a polypeptide hormone that have functions in promoting neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. It's actions appear to be restricted to the nervous system. Ciliary neurotrophic factor (CNTF) has biological effects through the activation of a multi- subunit receptor complex, consisting of an extracelluar CNTF binding subunit (CNTFα) and two transmembrane signal transduction proteins: glycoprotein gp130 and LIF receptor. CNTF is considered as a potent survival factor of neurons and oligodendrocyteands may be relevant in reducing tissue destruction during inflammatory attacks. CNTF also is a survival factor for neurons of the peripheral sensory sympathetic and ciliary ganglia. It has been reported that CNTF could be an agent that has therapeutic potential and possibly induces differentiation of large multipolar ganglionic phenotype in a subset of progenitors.